纳米氧化锌对羊角月牙藻毒性效应及其在藻细胞内外的分布

发布时间：2019-07-06 16:56

【摘要】：为研究纳米氧化锌(ZnO NP)的毒性效应及其在细胞内外分布,以羊角月牙藻(Selenastrum capricornutum)为模型藻类,研究了不同浓度ZnO NP对羊角月牙藻生长、叶绿素含量、可溶性蛋白含量、超氧化物岐化酶(SOD)及过氧化物酶(POD)活性、丙二醛(MDA)含量及细胞内外ZnO NP含量变化。结果表明,ZnO NP对羊角月牙藻的生长抑制与处理浓度呈现正相关。在45 mg·L~(-1)ZnO NP暴露24 h后,其生长抑制率已达到95%。当ZnO NP处理藻细胞72 h后,羊角月牙藻细胞的叶绿素含量与处理浓度之间存在剂量-效应关系。低浓度(0.5 mg·L~(-1))ZnO NP处理后藻细胞可溶性蛋白质含量、SOD和POD活性明显下降,MDA含量升高,其产生的毒性效应高于高浓度组(5 mg·L~(-1)、45 mg·L~(-1))。细胞培养液溶出Zn2+量及藻细胞外吸附的ZnO NP量与ZnO NP处理浓度成正比,但是藻细胞内ZnO NP量与ZnO NP浓度没有相关性,胞内积累量基本维持不变。研究表明,各浓度组对藻细胞毒性的差异,不仅与细胞内Zn2+量有关,还与细胞外粘附的ZnO NP有关。
文内图片：

图片说明：ZnONP对羊角月牙藻细胞密度影响
[Abstract]:In order to study the toxic effect of nanometer zinc oxide (ZnO NP) and its distribution inside and outside the cell, the changes of growth, chlorophyll content, soluble protein content, (SOD) and peroxide (POD) activity, malondialdehyde (MDA) content and intracellular and extracellular ZnO NP content of different concentrations of ZnO NP on the growth, chlorophyll content, soluble protein content, malondialdehyde (MDA) content and intracellular and extracellular ZnO NP content of Cymnaria angustifolia were studied. The results showed that the growth inhibition of, ZnO NP was positively correlated with the treatment concentration. After exposure to 45 mg 路L ~ (- 1) ZnO NP for 24 h, the growth inhibition rate reached 95%. When algae cells were treated with ZnO NP for 72 h, there was a dose-effect relationship between chlorophyll content and treatment concentration. The soluble protein content, SOD and POD activity and MDA content of algae cells treated with low concentration (0.5 mg 路L ~ (- 1) ZnO NP) were significantly decreased and MDA content was increased, and the toxic effect was higher than that of high concentration group (5 mg 路L ~ (- 1), 45 mg 路L ~ (- 1). The amount of Zn2 dissolved in the cell culture medium and the amount of ZnO NP adsorbed by algae outside the cells were proportional to the concentration of ZnO NP treatment, but the amount of ZnO NP in algae cells was not correlated with the concentration of ZnO NP, and the intracellular accumulation remained basically unchanged. The results showed that the difference of cytotoxicity to algae in each concentration group was related not only to the amount of intracellular Zn2, but also to the extracellular adhesion of ZnO NP.
【作者单位】：广东工业大学环境科学与工程学院;太原师范学院生物系;
【基金】：国际科技合作专项子项目(2013DFG50150) 广东省科技计划项目(2015A3FC0023,2013B020600007) 山西省科技基础条件平台建设项目(2015091004-0102) 山西省教学改革项目(J2014092)
【分类号】：X171.5

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