荧光蛋白与超分辨显微成像

发布时间：2018-03-29 15:18

本文选题：显微　切入点：超分辨显微成像　出处：《光学学报》2017年03期

【摘要】：超分辨显微成像技术使细胞生物学进入到了一个全新的时代,但如何进一步提高超分辨显微成像技术的时空分辨率仍是光学领域需要解决的重要问题。目前为止几乎所有的超分辨显微成像技术都依赖于荧光探针,光调控荧光蛋白作为一类特殊的荧光探针,可以被不同波长的激发光所激活,产生随机或者特殊结构样式的信号。利用这些信息,透镜系统的空间分辨率得到了提高。通过总结光调控荧光蛋白的各类参数,从荧光探针入手,寻找进一步提高成像系统空间分辨率的方法与策略,为选取适当的荧光探针提供建议,并且阐述了荧光蛋白与超分辨显微成像技术之间的关系。
[Abstract]:Super-resolution microscopic imaging has ushered in a new era in cell biology. However, how to further improve the spatial and temporal resolution of super-resolution microscopic imaging is still an important problem in optical field. Up to now, almost all super-resolution microscopic imaging techniques depend on fluorescence probe. As a special fluorescent probe, photoluminescence proteins can be activated by excitation light of different wavelengths to produce random or special structural signals. The spatial resolution of the lens system has been improved. By summing up the various parameters of the fluorescent protein regulated by light and starting with the fluorescence probe, the methods and strategies for further improving the spatial resolution of the imaging system are found. The relationship between fluorescent protein and superresolution microimaging technique is discussed.
【作者单位】：中国科学院核酸生物学重点实验室;中国科学院大学生命科学学院;
【基金】：国家自然科学基金(31370851) 国家973计划(2016YFA0501500)
【分类号】：TP391.41;O657.3

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