布伦登卢普沙门菌耐药性与毒力基因分析

发布时间：2017-12-26 16:05

本文关键词：布伦登卢普沙门菌耐药性与毒力基因分析　出处：《天津医药》2016年11期 　论文类型：期刊论文

【摘要】：目的研究临床分离的非伤寒沙门菌布伦登卢普血清型(布伦登卢普沙门菌)对抗菌药物的耐药性及其携带的毒力基因特征。方法收集2012—2014年每年4—10月天津市两家教学医院肠道门诊急性腹泻患者的临床资料,将急性腹泻患者粪便标本进行沙门菌分离培养、生化及PCR鉴定、血清学分型,对得到的布伦登卢普沙门菌进行抗菌药物敏感性检测、PCR扩增沙门菌毒力岛(SPI)1~5的代表性基因和SPI的调节基因。结果 3年共检测到非重复非伤寒沙门菌153株,其中8株(5.23%)为布伦登卢普沙门菌。8株inv A-PCR鉴定均呈阳性,对萘啶酸耐药率100%,对环丙沙星和左氧氟沙星中介耐药率100%,对其余检测的抗菌药物敏感;8株均检测到了SPI 1~5代表性基因和SPI调节基因(sit C、hil A、sse L、sif A、mgt C、sii E、sop B和pho P)。结论天津地区布伦登卢普沙门菌临床株对氟喹诺酮耐药并携带SPI 1~5毒力基因与调节基因,对公众健康构成潜在威胁,应持续开展相关监测与研究。
[Abstract]:Non typhoid Salmonella serotype Patrick Lupu objective to study the clinical isolates (Brendon Lupe Shamen Rand) of antibiotic resistance and virulence gene carrying characteristics. Methods collected each year from 2012 to 2014 4 patients with acute diarrhea intestinal clinic in Tianjin city in October the two teaching hospital clinical data, the feces from patients with acute diarrhea were Salmonella isolation, PCR identification, biochemical and serological typing, the Patrick Loup antimicrobial susceptibility of Salmonella detection, PCR amplification of Salmonella pathogenicity island (SPI) the representative of the regulatory gene 1~5 gene and SPI. The results of 3 years were detected in non repeated non typhoid Salmonella in 153 strains, including 8 strains (5.23%) for Salmonella Lupu patrick. Identification of 8 strains of inv A-PCR were positive, resistant to nalidixic acid 100%, 100% for ciprofloxacin and levofloxacin resistance rate of the intermediary, the detection of antimicrobial susceptibility; 8 strains were detected in the SPI 1~5 representative gene and SPI regulated genes (sit C, HIL A, SSE L, SIF A, Mgt C, SII E, SOP B and pho P). Conclusion Tianjin Lupu Patrick Salmonella isolates to fluoroquinolones resistance and carrying SPI 1~5 virulence genes and regulatory genes, constitutes a potential threat to public health, should continue to carry out the relevant monitoring and research.
【作者单位】：天津医科大学第二医院感染性疾病研究所;
【基金】：天津市卫生局科研基金资助项目(2013KZ112)
【分类号】：R446.5
【正文快照】： 在各种食源性病原体中,非伤寒沙门菌(Non-typhoidal Salmonella,NTS)造成的公共健康问题非常突出,在全球每年约导致9 380万人患急性胃肠炎和15.5万人死亡[1]。沙门菌毒力岛(SalmonellaPathogenicity Islands,SPI)是沙门菌染色体上的大片段DNA,可携带多种毒力基因,帮助其侵入人

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