ADRP基因在宗地花猪及其杂交猪不同组织中的表达分析

发布时间：2017-12-26 23:26

本文关键词：ADRP基因在宗地花猪及其杂交猪不同组织中的表达分析　出处：《基因组学与应用生物学》2016年08期 　论文类型：期刊论文

【摘要】：为研究ADRP基因在宗地花猪及其二元杂交猪不同组织中的表达规律,探讨该基因与脂肪含量的关系,试验以宗地花猪及其二元杂交猪为材料,采用实时荧光定量PCR技术,检测ADRP基因在心脏、肝脏、脾脏、肺脏、肾脏、小肠、背最长肌及脂肪中的相对表达量。结果表明,ADRP基因在宗地花猪及其杂交猪各组织中均有表达,不同组织之间及不同猪种之间的表达存在差异,表达丰度关系宗地花猪的为小肠脂肪肺脏肝脏心脏背最长肌脾脏肾脏,宗江二元猪的为脂肪小肠脾脏肺脏肾脏肝脏背最长肌心脏;显著性检验结果显示,宗江二元猪肝脏、脾脏、肺、小肠、背最长肌中ADRP基因的相对表达量显著高于宗地花猪的(p0.01),心脏、肾脏和脂肪组织中的表达量显著低于宗地花猪的(p0.01)。结果提示,ADRP基因在宗地花猪及其二元杂交猪不同组织中的差异表达可能与脂肪沉积有关。
[Abstract]:For the regular expression of ADRP gene in land and two yuan flower pig Hybrid Pigs in different tissues, to explore the relationship between the gene and the fat content of the test, to zongdihua pigs and two hybrid pigs as materials, using real-time quantitative PCR technique to detect ADRP gene relative expression quantity in the heart, liver, spleen, lung, kidney, small intestine, longissimus muscle and fat. The results showed that ADRP gene was expressed in each tissue flower pig and its crossbred pigs in the parcel, differences in expression between different tissues and between different breeds, the expression abundance relationship of zongdihua pigs for fatty liver heart lung spleen kidney longissimus muscle, Zongjiang pig intestine spleen for binary fatty liver lung kidney back the longissimus muscle heart; significant test results showed that the relative expression of ADRP Zongjiang binary pig liver, spleen, lung, intestine, longissimus muscle gene was significantly higher than that of zongdihua pigs (P0.01), the expression of heart, kidney and adipose tissue were significantly lower than that in zongdihua pigs (P0.01). The results suggest that the differential expression of ADRP gene in the different tissues of hybrids and two yuan hybrids may be related to the fat deposition.
【作者单位】：贵州大学动物科学学院;高原山地动物遗传育种与繁殖省部共建教育部重点实验室;贵州省紫云自治县畜牧兽医局;贵州省农业委员会;
【基金】：贵州省技厅农业攻关项目“贵州宗地花猪杂交配套体系的研究及推广应用”[黔科合NZ字(2012)3013] 紫云宗地花猪生态养殖技术集成与产业化示范项目[黔科合县市科拨计划(2012)7032]共同资助
【分类号】：S828
【正文快照】： Expression Analysis of ADRP Gene in Different Tissues from ZongdihuaPig and Bi-crossbreeding PigGu Liju1,2Yan Zhihong1,2*Lin Pengfei1,2Ren Liqun1,2Li Ping1,2Tian Songjun3Wei Xiaohong3Zhang Yun41 College of Animal Science,Guizhou University,Guiyang,550025

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