针刺对抑郁症大鼠海马5-HT、NE、BDNF水平和神经元细胞凋亡相关基因表达的影响

发布时间：2017-12-27 15:13

  本文关键词：针刺对抑郁症大鼠海马5-HT、NE、BDNF水平和神经元细胞凋亡相关基因表达的影响　出处：《中华中医药杂志》2016年08期 　论文类型：期刊论文

  更多相关文章： 针刺 单胺类神经递质 脑源性神经因子 神经元细胞凋亡 抑郁症 大鼠 海马

【摘要】：目的:探讨针刺对神经元细胞的保护作用及其可能分子机制。方法:侧脑室注射鹅膏蕈氨酸建立神经元损伤大鼠模型;造模成功后,给予针刺和药物干预,观察大鼠的行为学变化、海马神经元细胞形态学改变;检测单胺类神经递质(5-HT、NE)含量的变化、脑源性神经因子(BDNF)和凋亡蛋白Bax/Bcl-2的变化。结果:与空白组比较,模型组表现出运动量减少(P0.05)、食欲和对新环境的适应能力下降(P0.05)、神经元细胞数目减少、核内空泡增多、连接松散、5-HT和NE含量显著下降(P0.05)、Bax/Bcl-2比值升高(P0.05)。针刺和药物干预后,大鼠运动量、食欲和对新环境的适应能力得到加强(P0.05),神经元细胞数目增加,连接松散的形态得到改善,核内空泡数量减少,5-HT、NE和BDNF含量均升高(P0.05),Bax/Bcl-2比值降低(P0.05)。结论:针刺对神经元的保护作用可能是通过提高5-HT、NE和BDNF的含量,减少神经元细胞凋亡起作用的。
[Abstract]:Objective: To explore the protective effect of acupuncture on neuron cells and its possible molecular mechanism. Methods: intracerebroventricular injection of ibotenic acid to establish neuron injury model in rats; after the success of the model, given acupuncture and drug intervention, to observe the behavioral changes of rats, hippocampal neuron cell morphology; Determination of monoamine neurotransmitters (5-HT, NE), the content changes of brain-derived neurotrophic factor (BDNF) changes and apoptosis protein Bax/Bcl-2. Results: compared with the blank group, the model group showed a reduction in the amount of movement (P0.05), decreased appetite and ability to adapt to the new environment (P0.05), reduced the numbers of neurons, increased, nuclear vacuoles in loose connections, 5-HT and NE were significantly decreased (P0.05), Bax/Bcl-2 ratio (P0.05). Acupuncture and drug intervention, rats weight, appetite and ability to adapt to the new environment has been strengthened (P0.05), the increase in the number of neurons, loosely connected structure is improved, the number of nuclear vacuoles reduced, 5-HT, NE and BDNF were significantly increased (P0.05), Bax/Bcl-2 ratio (P0.05). Conclusion: the protective effect of acupuncture on neurons may be mediated by increasing the content of 5-HT, NE and BDNF and reducing neuronal cell apoptosis.
【作者单位】： 广东省中医院;香港浸会大学中医药学院;广州中医药大学;中国循证医学中心;广东省中医药科学院;
【基金】：国家自然科学基金青年科学基金项目(N o.81303041) 中国博士后科学基金面上项目(No.2012M511784) 广东省教育厅高校优秀青年创新人才培养计划项目(No.2012LYM_0043) 国家教育部高等学校博士学科点新教师类专项科研基金(No.20124425120005) 中国博士后基金特别资助项目(No.2013T60793) 广东省人社厅资助广州中医药大学博士后研究人员科研基金(No.BBK429122K19)~~
【分类号】：R245
【正文快照】： Funding:Young Scientists Fund of the National Natural Science Foundation of China(No.81303041),General Programof China Postdoctoral Science Foundation(No.2012M511784),Science Foundation of the Outstanding Young InnovativePersonnel of Department of Educat，

本文编号：1342214