低钾胁迫对烟草幼苗叶中基因表达的影响

发布时间：2017-12-27 20:23

本文关键词：低钾胁迫对烟草幼苗叶中基因表达的影响　出处：《核农学报》2016年07期 　论文类型：期刊论文

【摘要】：为探索在低钾胁迫下烟草幼苗基因表达谱的变化,对烟草幼苗进行了0、6、12、24 h低钾胁迫,并利用基因芯片技术分析了烟草幼苗基因表达谱的变化。结果表明,与对照相比,处理组基因表达量变化在2倍以上的差异表达基因共有3 790个。这些差异表达基因所行使的功能多达18种,包括抗氧化活性、催化活性、酶调活性,并参与应激反应、发育过程以及代谢过程等。在差异表达基因中,涉及到响应非生物刺激相关差异表达基因有417个(281个上调、136个下调)、光合作用基因有21个(10个上调、11个下调)、光合色素合成相关基因9个(6个上调、3个下调)以及植物激素代谢相关基因6个(5个上调、1个下调)。研究结果可为烟草钾营养的分子机制研究提供借鉴。
[Abstract]:In order to explore the change of gene expression profile of tobacco seedlings under low potassium stress, tobacco seedlings were subjected to 0, 6, 12, and 24 h low potassium stress, and the gene expression profiles of tobacco seedlings were analyzed by gene chip technology. The results showed that, compared with the control, there were 3790 differentially expressed genes with more than 2 times the change of gene expression in the treatment group. These differentially expressed genes can function as many as 18 kinds, including antioxidant activity, catalytic activity, enzyme activity, and participate in stress response, development process and metabolic process. Differences in gene expression in the response to abiotic stress related gene 417 (281 up-regulated and 136 down regulated), photosynthesis 21 genes (10 up-regulated and 11 down regulated), photosynthetic pigment synthesis related gene 9 (6 up-regulated and 3 down regulated) and plant hormone 6 metabolism related genes (5 up-regulated and 1 down regulated). The results can be used for reference in the study of molecular mechanism of potassium nutrition in tobacco.
【作者单位】：四川农业大学农学院;
【基金】：中国烟草总公司科技重大专项[110201401007(JY-07)]
【分类号】：S572
【正文快照】： 钾是植物必需大量元素之一[1]。在农业生产中,缺钾会阻碍农作物进行光合作用,导致产量及品质降低[2-3]。烟草是重要的经济作物,也是喜钾作物[4-5]。研究表明,土壤缺钾将导致烟草产量降低、烟叶含钾量与品质下降[6-7]。我国植烟土壤普遍缺钾,制约了我国烟叶品质的提高[8-9]。目

【相似文献】