非洲菊转基因毛状根诱导系统的建立

发布时间：2017-12-28 13:03

本文关键词：非洲菊转基因毛状根诱导系统的建立　出处：《植物生理学报》2016年09期 　论文类型：期刊论文

【摘要】：非洲菊(Gerbera hybrida)是研究复杂花序发育的模式植物,目前存在转基因效率不高的问题。为建立非洲菊转基因毛状根诱导系统,本文探究了不同非洲菊品种、发根农杆菌(Agrobacterium rhizogenes)菌种、外植体以及侵染和共培养时间等因素对非洲菊毛状根诱导的影响。实验所获得的最佳诱导条件为:非洲菊品种选取‘玲珑’,菌种选取发根农杆菌K599,外植体选取生长21~28 d的叶片叶基部,农杆菌菌液浓度OD_(600)=0.6时侵染20 min,共培养2 d;在此培养条件下,生根率高达81.14%。进一步利用所建立的转化系统,成功获得了35S::At HBI1-GUS转基因毛状根,由于At HBI1蛋白具有促进细胞伸长的功能,与对照相比,转基因毛状根的生长速率显著提高。本文所建立的非洲菊转基因毛状根诱导体系将为非洲菊基因功能的研究,特别是生长及代谢相关基因的研究提供技术手段。
[Abstract]:Gerbera (Gerbera hybrida) is a model plant of complex inflorescence development, the existing problem of high transfection efficiency. For the establishment of transgenic Gerbera hairy root induction system, this paper explores the different Gerbera varieties of Agrobacterium rhizogenes, (Agrobacterium rhizogenes) strains, explant and infection and co cultivation time and other factors to induce hairy roots of gerbera. The optimal induction conditions obtained by the experiment is: "exquisite", selected varieties of Gerbera jamesonii selected strains of Agrobacterium rhizogenes K599, explant selection of leaf base growth of 21~28 D, OD_ of bacterial concentration (600 =0.6) from 20 min, 2 D of co culture; under these conditions, the rooting rate reached 81.14%. The transgenic hairy roots of 35S:: At HBI1-GUS were successfully obtained by using the transformation system. Because At HBI1 protein has the function of promoting cell elongation, the growth rate of transgenic hairy roots is significantly higher than that of control. This paper established the induction system of Gerbera transgenic hairy roots for the research of Gerbera genes, especially in the provision of technical means to study the growth and metabolism related genes.
【作者单位】：华南师范大学生命科学学院广东省植物发育生物工程重点实验室;
【基金】：国家自然科学基金(31372099)~~
【分类号】：S682.11
【正文快照】： 收稿2016-08-05修定2016-09-04非洲菊(Ger bera hybrida)属菊科(Asteraceae)大丁草属多年生草本植物,其头状花序上存在着舌状花、过渡花及盘状花3种不同类型的小花,是研究花瓣形态建成调控机制的理想材料,目前在国际上已被作为研究复杂花序的模式植物(Meng和Wang 2004;Broholm

【相似文献】