紫花苜蓿MsHST基因克隆及拟南芥转化

发布时间：2017-12-28 23:12

  本文关键词：紫花苜蓿MsHST基因克隆及拟南芥转化　出处：《草地学报》2016年06期 　论文类型：期刊论文

  更多相关文章： 紫花苜蓿 MsHST 拟南芥 转基因

【摘要】：运用RT-PCR法从紫花苜蓿(Medicago sativa)中成功克隆出长度约1100bp的尿黑酸法呢基转移酶基因MsHST,采用qRT-PCR的方法,分析MsHST基因的表达特征,结果显示MsHST在所有组织中均有表达,但在叶片中表达量最高,根中最低;随叶片的衰老,表达量降低。且构建了超表达载体pBI-MsHST,并获得拟南芥(Arabidopsis thaliana)转基因植株,经PCR,RT-PCR和GUS染色检测转基因植株,初步证实目的基因在拟南芥中表达。
[Abstract]:By using the method of RT-PCR from Alfalfa (Medicago sativa) successfully cloned homogentisic acid method with length of about 1100bp farnesyl transferase gene MsHST, qRT-PCR method was used to analyze the features of MsHST gene expression, the results showed that MsHST in all tissues, but the highest expression level in leaves and roots with the lowest; leaf senescence, decreased expression. The overexpression vector pBI-MsHST was constructed, and the Arabidopsis thaliana transgenic plants were obtained. The transgenic plants were detected by PCR, RT-PCR and GUS staining, and preliminarily confirmed that the target gene was expressed in Arabidopsis thaliana.
【作者单位】： 内蒙古农业大学农学院;中国农业科学院北京畜牧兽医研究所;
【基金】：十二五国家科技支撑子课题“紫花苜蓿品质性状相关基因的挖掘与功能分析”(2014CB138703-2) 中国农业科学院北京畜牧兽医研究所基本科研业务费项目(2014ywf-zd-2)资助
【分类号】：S541.9;Q943.2
【正文快照】： 尿黑酸法呢基转移酶(Homogentisate solanesyl-transferase,HST)是高等植物体内普遍存在的一种不可或缺的酶,他对植物生长发育的调控及光系统II(PSII)传递电子的辅因子PQ的合成发挥着不可替代的重要作用[1,2]。除此之外,尿黑酸法呢基转移酶还能参与植物代谢中类胡萝卜素与ABA，

本文编号：1347815