肺形侧耳变温结实相关基因Ppcsl-1的克隆及功能预测

发布时间：2017-12-28 20:43

本文关键词：肺形侧耳变温结实相关基因Ppcsl-1的克隆及功能预测　出处：《菌物学报》2016年08期 　论文类型：期刊论文

【摘要】：CSL(CBF1/RBP-Jκ/suppressor of hairless/LAG-1)转录因子家族在真菌发育和细胞分化过程中扮演重要角色。前期研究已构建了肺形侧耳变温结实相关消减杂交文库,并从中筛选到一个代表csl基因部分序列的EST。通过TAIL PCR(thermal asymmetric interlaced PCR)技术克隆了该基因(Pleurotus pulmonarius csl-1,简写为Ppcsl-1),并利用RACE(rapid-amplification of c DNA ends)技术获得该基因的c DNA全长。Ppcsl-1 c DNA全长2 991bp,编码一个996个氨基酸组成的蛋白(命名为Pp CSL-1)。进化分析显示在担子菌CSL中,Pp CSL-1与糙皮侧耳Pleurotus ostreatus CSL(PoC SL)亲缘关系最近。荧光定量检测结果表明Ppcsl-1在菌丝经过5℃12h冷处理之后的表达量最高,表明其有可能被冷刺激诱导表达并在开启子实体形成的过程中起重要作用。
[Abstract]:The CSL (CBF1/RBP-J kappa /suppressor of hairless/LAG-1) transcription factor family plays an important role in the process of fungal development and cell differentiation. In the previous study, the lung shaped lateral ear variable temperature and seed setting related subtractive hybridization library had been constructed, and a EST representing the partial sequence of CSL gene was screened from it. The TAIL PCR (thermal asymmetric interlaced PCR) technology was used to clone the gene (Pleurotus pulmonarius csl-1, short for Ppcsl-1), and the full length of the gene was obtained by the technology of RACE. Ppcsl-1 C DNA has a total length of 2 991bp and encodes a protein of 996 amino acids (named Pp CSL-1). Phylogenetic analysis showed that CSL Pp CSL-1 in basidiomycetes, Pleurotus ostreatus CSL and Pleurotus ostreatus (PoC SL) had the closest relationship. Fluorescence quantitative analysis showed that Ppcsl-1 had the highest expression level after cold treatment at 5 12h, indicating that it could be induced by cold stimulation and played an important role in the formation of fruiting bodies.
【作者单位】：浙江师范大学化学与生命科学学院;浙江省农业科学院园艺研究所;
【基金】：浙江省农业新品种选育重大科技专项-食用菌新品种选育(2012C12911) 国家自然科学基金(31401930) 浙江省自然科学基金(LQ13C150005)~~
【分类号】：Q949.32
【正文快照】： 环境(如温度),是影响食用菌结实出菇的重了细胞内多个信号转导途径的参与。转录因子作要外在因素。一些食用菌必需经过低温刺激才能开为信号转导系统的最初效应蛋白,在生物生长发启由营养菌丝到子实体的转变,这种现象称为“变育和器官分化过程中起到重要作用。CSL(CBF1/温结实

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