荔枝椿平腹小蜂触角味觉受体基因的鉴定与表达分析

发布时间：2018-01-01 22:41

本文关键词：荔枝椿平腹小蜂触角味觉受体基因的鉴定与表达分析　出处：《吉林农业大学学报》2017年03期 　论文类型：期刊论文

【摘要】：昆虫具有极其敏锐的味觉感受系统,昆虫的味觉系统对其取食选择、交配和产卵等多种行为反应起到重要的作用。荔枝椿平腹小蜂(Anastatus japonicus)作为膜翅目中一种重要的寄生蜂,具有防治荔枝椿象的作用。研究对荔枝椿平腹小蜂触角进行转录组测序,从数据库进行筛选鉴定得到5个味觉受体基因。借助RT-PCR对荔枝椿平腹小蜂的雌性触角、雄性触角、胸、腹和腿部味觉受体的表达量进行比较,发现Ajap GR58,Ajap GR49和Ajap GR64f在成虫的各组织部位表达量一致并均较低,而Ajap GR38和Ajap GR2的表达量相对较高,同时,Ajap GR38只在雌性触角以及胸部特异性表达,Ajap GR2则是在雌性触角、雄性触角、胸部和腹部均有表达,而腿部却不表达。通过系统发育树可以看出,Ajap GR58和Ajap GR49同源,与DmelGR33a,Dmel GR43a聚在一起,Ajap GR38与Dmel GR28同源,与Dmel GR66a聚在一起,说明Ajap GR58、Ajap GR49以及Ajap GR38处于苦味受体家族中,可能也行使苦味感知功能。Ajap GR64f与Dme GRl5,DmelGR64f同源并与Dmel GR64a聚在一起,同时,Ajap GR2与Dmel GR64a,Dmel GR64b,Dmel GR64c,Dmel GR64f以及Dmel GR5所形成的一类并聚在一起,说明Ajap GR64f和Ajap GR2处于甜味受体家族中,可能也行使甜味感知功能。
[Abstract]:Insects have a very sharp taste sense system, the taste system of insects to feed on the choice. Mating and oviposition play an important role. Anastatus japonicus is an important parasitic wasp in Hymenoptera. In this study, transcriptome sequencing of the antennae of Toona chinensis was carried out. Five taste receptor genes were screened and identified from the database. The expression levels of female antennae, male antennae, chest, abdomen and leg taste receptors were compared by RT-PCR. It was found that the expression of Ajap GR49 and Ajap GR64f in Ajap GR58 was consistent and low in all tissues of adults. The expression of Ajap GR38 and Ajap GR2 was relatively high, and the expression of Ajap GR38 was only specific in female antennae and chest. Ajap GR2 is expressed in the female antennae, male antennae, chest and abdomen, but not in the legs, as can be seen from the phylogenetic tree. Ajap GR58 and Ajap GR49 are homologous to DmelGR33aMel GR43a and Ajap GR38 is homologous to DmelGR 28. Together with Dmel GR66a, Ajap GR58 Ajap GR49 and Ajap GR38 are in the bitter receptor family. Ajap GR64f and Dme GRl5DmelGR64f may also perform bitter taste perception function and gather with Dmel GR64a at the same time. Ajap GR2 and Dmel GR64a Dmel GR64b Dmel GR64c. The combination of Dmel GR64f and Dmel GR5 suggests that Ajap GR64f and Ajap GR2 are in the sweet receptor family. May also exercise sweet sense function.
【作者单位】：东北师范大学吉林省动物资源保护与利用重点实验室吉林省鸟类生态与保护遗传工程实验室;吉林农业大学现代化研究所;
【基金】：吉林省自然科学基金项目(20150101068JC)
【分类号】：S476.3
【正文快照】： 引文格式:李婧,张俊杰,王寅亮,等.荔枝椿平腹小蜂触角味觉受体基因的鉴定与表达分析[J].吉林农业大学学报,2017,39(3):292-298,312.***通信作者tatory receptors of Anastatus japonicus female antennae,male antennae,thorax,abdomen and legwere compared.The results show

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