降低日粮蛋白质含量并补充氨基酸对泌乳母猪生产性能、乳腺吸收效率和转运体基因表达的影响

发布时间：2018-01-02 16:18

本文关键词：降低日粮蛋白质含量并补充氨基酸对泌乳母猪生产性能、乳腺吸收效率和转运体基因表达的影响　出处：《中国饲料》2016年15期 　论文类型：期刊论文

更多相关文章： 氨基酸 效率基因 哺乳期 母猪 转运载体

【摘要】：为验证降低日粮蛋白质水平并补充晶体氨基酸是否可以提高氨基酸用于泌乳母猪产奶的使用效率,试验选用3种日粮,蛋白质含量分别为9.5%(不足型)、13.5%(理想型)和17.5%(标准型)饲喂泌乳母猪,各日粮氨基酸水平相同。检测乳腺氨基酸动静脉浓度差(A-V)、氨基酸运输效率(A-V/A×100)、氨基酸转运载体和乳蛋白基因的转录丰度。在第7和18天,收集采食后动脉、乳腺静脉血液和乳腺组织样品。使用定量PCR测定氨基酸转运载体b0,+AT(SLC7A9)、Y+LAT2(SLC7A6)、ATB0,+(SLC6A14)、CAT-1(SLC7A1)、CAT-2b(SLC7A2)以及β酪蛋白(CSN2)和α乳清蛋白(LALBA)的转录丰度。结果表明,随着蛋白质含量从不足型到标准型逐渐增加,仔猪日增重呈曲线上升(线性和二次方,P0.03)。第7天,随粗蛋白质含量增加,赖氨酸和精氨酸A-V、运输效率呈二次方增加(P0.05)。第18天,随粗蛋白质含量增加,赖氨酸A-V呈增加趋势(线性,P=0.08)。第7天,随着粗蛋白质含量增加,异亮氨酸和缬氨酸A-V增加(呈线性,分别为P=0.05和P=0.08)。第18天,异亮氨酸和缬氨酸浓度增加(呈线性,分别为P=0.04和P=0.07)。第7天,血液支链AA(BCAA):赖氨酸(Lys)比值呈二次方降低(P0.05)。SLC7A9、SLC7A6、SLC6A14、SLC7A1、SLC7A2、CSN2和LALBA的基因表达不受日粮影响。总之,日粮蛋白质含量从17.5%下降到13.5%,并补充晶体氨基酸并不影响仔猪日增重,氨基酸转运载体或乳蛋白基因的表达,但可以增加泌乳第7天乳腺运输效率和赖氨酸与精氨酸动静脉浓度差。这种增加与血液BCAA∶Lys比值下降有关,表明氨基酸运输至乳腺细胞时,阳离子型AA和BCAA之间存在竞争机制。
[Abstract]:In order to verify whether the decrease of dietary protein level and crystal amino acid can improve the efficiency in the use of amino acids for lactating sow milk, 3 test diets with protein content were 9.5%, 13.5% (short) (ideal) and 17.5% (standard) fed lactating sows, the same dietary amino acid level detection. Breast amino acid arteriovenous concentration difference (A-V), amino acid transport efficiency (A-V/A * 100), transcript abundance of amino acid transporters and milk protein gene. In the seventh and 18 days, collected after feeding artery venous blood, breast and breast tissue samples. The determination of amino acid transporter B0 using quantitative PCR, +AT (SLC7A9). Y+LAT2 (SLC7A6), ATB0 + (SLC6A14), CAT-1 (SLC7A1), CAT-2b (SLC7A2) and beta casein (CSN2) and alpha whey protein (LALBA) of the transcript abundance. The results show that with the content of protein from deficiency to the standard gain of piglets increased gradually. The heavy curve rises (linear and two, P0.03). The seventh day, with increasing the content of crude protein, lysine and arginine transport efficiency was two A-V, increase (P0.05). The eighteenth day, with increasing the content of crude protein, lysine A-V increased (linear, P=0.08) at seventh days, with the increase of the content of crude protein, isoleucine and valine (A-V increased linearly, respectively P=0.05 and P=0.08). The eighteenth day, increasing the concentration of isoleucine and valine (linear, respectively P=0.04 and P=0.07). The seventh day, blood branched AA (BCAA): lysine (Lys) ratio was two reduce your time (P0.05).SLC7A9, SLC7A6, SLC6A14, SLC7A1, SLC7A2, CSN2 and LALBA gene expression is not affected by the dietary effects. In short, the dietary protein content decreased from 17.5% to 13.5%, adding crystalline amino acid does not affect the weight gain of piglets, expression carrier or milk protein gene amino acid, but can be increased seventh lactation The transport efficiency of the mammary gland and the difference of lysine and arginine arteriovenous concentration were related to the decrease of BCAA to Lys ratio. It indicates that there is a competition mechanism between the cationic AA and BCAA when the amino acid is transported to the mammary gland cells.

【作者单位】：密歇根州立大学动物科学学院;墨西哥州Montecillo研究生院;德克萨斯州农工大学动物科学和营养学部;密歇根州立大学渔业和野生动物系;美国密歇根州立大学大型动物临床科学系;
【分类号】：S828.5
【正文快照】： 为了使猪更好地利用日粮蛋白质,越来越多酸利用率在细胞水平上的作用机制还不是很清的研究关注最佳日粮氨基酸(AA)水平(NRC,楚。日粮AA胞内可利用性是由位于细胞膜上允2012)。有研究表明,日粮AA过量或不足引起的许氨基酸进入细胞内的蛋白质载体控制(Bro壵r,AA不平衡可导致哺乳

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