基于cDNA-AFLP技术分析不同种源和地域太子参基因差异表达

发布时间：2018-01-02 15:12

本文关键词：基于cDNA-AFLP技术分析不同种源和地域太子参基因差异表达　出处：《天然产物研究与开发》2016年02期 　论文类型：期刊论文

【摘要】：研究不同种源和地域太子参的基因差异表达,分离并鉴定相关差异基因。采用c DNA-AFLP技术分析2个不同种源和地域太子参差异表达基因。筛选6个引物组合进行扩增,从同种源不同地域及同地域不同种源太子参中分别获得44、38条差异表达转录衍生片段(Trivially distributed file system,TDFs),对差异片段进行克隆、序列测定,分别得到27、25个TDFs核苷酸序列。经过BLASTX程序比对,分别有19、16个TDFs有其对应的显著同源序列,其中有7、6个TDFs为已知功能的蛋白,这些蛋白主要参与植物的生长发育、抵御病虫害、提高植物抗非生物胁迫的能力。本研究为揭示太子参药材品质形成的分子机制提供基础资料。
[Abstract]:To study the gene differential expression of Pseudostellaria heterophylla from different provenances and regions. Two differentially expressed genes from different provenances and regions of Pseudostellaria heterophylla were analyzed by c DNA-AFLP technique. Six primer combinations were screened for amplification. 44 were obtained from the same region and different provenances of the same region. 38 differentially expressed distributed file derived fragments were cloned and sequenced. 27 and 25 TDFs nucleotide sequences were obtained respectively. After BLASTX program alignment, there were 19 and 16 TDFs corresponding significant homologous sequences respectively, of which 7 were homologous. Six TDFs are known functional proteins, which are mainly involved in plant growth and development, resistant to pests and diseases. This study provides basic information for revealing the molecular mechanism of quality formation of Radix Pseudostellariae.
【作者单位】：南京中医药大学;
【基金】：国家自然科学基金(81274016;81473312) 江苏高校优势学科建设工程(ysxk-2014)
【分类号】：S567.53
【正文快照】： 太子参为大宗常用中药材,系石竹科假繁缕属植物孩儿参Pseudostellaria heterophylla(Miq.)Paxex Pax et Hoffm.的干燥块根[1],具益气健脾、润肺生津之传统功效及保护心肌功能、促进免疫、抗应激、治疗糖尿病、止咳等药理作用,临床上用于脾虚体倦、食欲不振、病后虚弱、气阴不

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