茄子MnSOD基因的克隆及表达分析

发布时间：2018-01-02 14:08

本文关键词：茄子MnSOD基因的克隆及表达分析　出处：《植物生理学报》2016年10期 　论文类型：期刊论文

【摘要】：以茄子品种‘禾线’为材料,采用同源克隆方法从茄子中克隆获得一个MnSOD基因,其开放阅读框全长为687 bp,编码228个氨基酸,将其命名为SmMnSOD。序列分析表明,SmMnSOD与辣椒MnSOD序列相似性达到91%,与番茄MnSOD序列相似性达到84%。预测该蛋白质分子量为25.63 k Da,蛋白等电点为8.46。亚细胞定位结果显示,MnSOD蛋白定位于线粒体。实时荧光定量PCR表明,SmMnSOD在不同组织器官中均有表达,但在叶片中表达量最多,其次为花瓣和根,果肉中最少。在Na Cl胁迫下,SmMnSOD表达量呈现先上升后下降趋势;聚乙二醇胁迫抑制了SmMnSOD的表达;外源脱落酸胁迫下,SmMnSOD的表达量是波动变化的;低温胁迫极显著地抑制了SmMnSOD的表达。实验结果证明,SmMnSOD主要在茄子叶片、花瓣和根中表达,在线粒体中发挥功效,可能与茄子抵御渗透性胁迫和干旱胁迫相关。
[Abstract]:A MnSOD gene was cloned from eggplant by homologous cloning method. Its open reading frame was 687bp, encoding 228 amino acids. It was named SmMnSOD.The sequence analysis showed that the similarity between SmMnSOD and pepper MnSOD sequence was 91%. The similarity of MnSOD sequence with tomato was 84. The predicted molecular weight of the protein was 25.63 K Da.The isoelectric point of the protein was 8.46. The subcellular localization results showed. MnSOD protein was located in mitochondria. Real-time quantitative PCR showed that SmMnSOD was expressed in different tissues and organs, but most in leaves, followed by petals and roots. The expression of SmMnSOD increased first and then decreased under NaCl stress. The expression of SmMnSOD was inhibited by polyethylene glycol stress. The expression of SmMnSOD fluctuated under exogenous abscisic acid stress. The results showed that SmMnSOD was mainly expressed in leaves, petals and roots of eggplant, and played an important role in mitochondria. It may be related to resistance of eggplant to osmotic stress and drought stress.
【作者单位】：上海交通大学农业与生物学院;
【基金】：国家自然科学基金(31301770和31471870) 上海市科技兴农项目[沪农科种字(2013)第5号]~~
【分类号】：S641.1
【正文快照】： 收稿2016-06-02修定2016-09-26正常情况下,活性氧(reactive oxygen species,ROS)在植物体内的产生和清除处于动态平衡,然而环境胁迫造成活性氧代谢失衡,导致活性氧的大量积累,对植物产生毒害作用,严重时引起细胞的死亡。超氧阴离子(O2ˉ·)是活性氧中的一种,O2ˉ·应及时被清

【相似文献】