云南汉族γ-干扰素受体基因多态性与动脉粥样硬化斑块稳定性的相关性

发布时间：2018-01-02 10:08

本文关键词：云南汉族γ-干扰素受体基因多态性与动脉粥样硬化斑块稳定性的相关性　出处：《重庆医学》2016年33期 　论文类型：期刊论文

【摘要】：目的探讨云南汉族γ-干扰素受体(IFNGR)的两个氨基酸位点Val14Met和GIn64Arg多态性与动脉粥样硬化(AS)斑块稳定性的相关性。方法收集该院2014年3月至2015年3月收治的AS斑块不稳定的患者作为观察组,而同期入院的AS斑块稳定/无斑块的患者为对照组。采集患者外周静脉血,提取基因组DNA,通过聚合酶链反应(PCR)产物直接测序法检测IFNGR1Vall4Met和IFNGR2Gln64Arg位点的基因型,采用DNAStar、GeneTool软件分析测序结果,用流式细胞术检测患者血浆细胞因子[γ-干扰素(IFN-γ)]的水平。结果 204例当地汉族患者列入研究,其中观察组109例,年龄(76.89±12.08)岁;对照组95例,年龄(65.99±16.32)岁。IFNGR1Vall4Met位点在观察组和对照组中均未发现多态性改变。IFNGR2Gln64Arg在观察组AA基因型频率为51.95%(40/77),AG基因型频率为53.06%(52/98),GG基因型频率为58.62%(17/29);对照组AA基因型频率为48.05%(37/77),AG基因型频率为46.94%(46/98),GG基因型频率为41.38%(12/29),卡方检验P=0.824,IFNGR2基因型AA、AG和GG与AS斑块稳定性没有关系;观察组IFNGR2A等位基因频率为52.38%(132/252),G等位基因频率为55.13%(86/156),对照组A等位基因频率为47.62%(120/252),G等位基因频率为44.87%(70/156),卡方检验校正P=0.661,IFNGR2等位基因A和G与AS斑块稳定性没有关系。经Hardy-Weinberg遗传平衡检验,该样本人群该基因频率符合遗传平衡法则。观察组血浆中IFN-γ水平为(4.60±1.91)ng/mL,对照组为(4.88±2.10)ng/mL,差异无统计学意义(P=0.318);血浆IFN-γ水平与AS斑块稳定性无相关性(P=0.308)。结论 IFNGR基因多态性不能作为AS斑块稳定性的预警指标。
[Abstract]:Objective to investigate the Yunnan Han interferon gamma receptor (IFNGR) two amino acid sites Val14Met and GIn64Arg polymorphism and atherosclerotic plaque stability (AS) correlation. Methods in our hospital from March 2014 to March 2015 were AS plaque instability patients as the observation group, while AS admitted plaque stability / no plaque of patients as control group. Patients with peripheral venous blood collection, extract genomic DNA by polymerase chain reaction (PCR) genotype, IFNGR1Vall4Met and IFNGR2Gln64Arg were detected by DNAStar sequencing, sequencing analysis results of GeneTool software, flow cytometry was used to detect plasma cytokines in patients with [interferon gamma (IFN- y)] level. Results 204 cases of local Han patients included in the study, 109 cases in the observation group, age (76.89 + 12.08) years; 95 cases in the control group, age (65.99 + 16.32).IFNGR1Vall4Met sites in the view Observation group and control group were not found in.IFNGR2Gln64Arg polymorphism in the observation group AA genotype frequency was 51.95% (40/77), AG genotype frequency was 53.06% (52/98), GG genotype frequency was 58.62% (17/29); control group AA genotype frequency was 48.05% (37/77), AG genotype frequency was 46.94% (46/98), GG genotype frequency was 41.38% (12/29), chi square test P=0.824, IFNGR2 genotype AA, AG and GG AS and plaque stability Never mind; the observation group IFNGR2A allele frequency was 52.38% (132/252), G allele frequency was 55.13% (86/156), the control group A allele the frequency of 47.62% (120/252), G allele frequency was 44.87% (70/156), chi square test corrected P=0.661, IFNGR2 allele and A G and AS Hardy-Weinberg. The stability of plaque Never mind genetic equilibrium test, the gene frequency of the sample population with genetic equilibrium law. Observe the level of plasma for IFN- gamma (4. 60 + 1.91) ng/mL, the control group was (4.88 + 2.10) ng/mL, the difference was not statistically significant (P=0.318). There was no correlation between plasma IFN- gamma level and the stability of AS plaque (P=0.308). Conclusion IFNGR gene polymorphism can not be used as an early warning indicator for the stability of AS plaque.

【作者单位】：云南省第一人民医院内干科;云南省第一人民医院基研室;
【分类号】：R543.5
【正文快照】： 动脉粥样硬化(atherosclerosis,AS)是一种常见多发疾病,其斑块的不稳定导致斑块破裂、血栓形成及血管阻塞,是急性心、脑血管事件的主要原因,极大的威胁着人民群众的生命健康,防治AS已成为当今医学科学研究的重大课题。γ-干扰素(IFN-γ)是多功能细胞因子,是巨噬细胞、自然杀伤

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