糖化LDL对人脐静脉血管内皮细胞SOD活性及基因表达的影响

发布时间：2018-01-03 20:32

本文关键词：糖化LDL对人脐静脉血管内皮细胞SOD活性及基因表达的影响　出处：《同济大学学报(医学版)》2016年06期 　论文类型：期刊论文

【摘要】：目的观察糖化低密度脂蛋白(gly-LDL)对人血管内皮细胞生长及抗氧化相关因子的影响,探讨糖尿病患者血管内皮损伤与gly-LDL的相关性及可能机制。方法用完全培养基及不同浓度(G1组0.06 mg/L,G2组0.12 mg/L,G3组0.24 mg/L)的gly-LDL处理人脐静脉血管内皮细胞(HUVECs)24 h、48 h及72 h,设对照组(Ctr),取细胞培养上清液检测SOD和MDA水平,同时用细胞划痕实验观察gly-LDL对内皮细胞迁移功能的影响,用实时半定量PCR(realtime quantitative PCR,RT-q PCR)方法测定Mn-SOD和e NOS的mRNA的表达情况。结果与对照组相比不同浓度的gly-LDL均可抑制血管内皮细胞的迁移功能。较低或较高浓度的gly-LDL作用于人脐静脉内皮细胞时,随时间的增加SOD的活性及MDA的含量是升高的;中间浓度的gly-LDL作用于人脐静脉内皮细胞时,随时间的增加SOD活性是逐渐下降的,MDA含量是先增高后降低的。各组Mn-SOD的mRNA表达水平48 h和72 h均下调,e NOS的表达水平于24 h和48 h均下调,72 h时上调。结论 Gly-LDL与糖尿病患者血管并发症可能存在一定的相关性,但不能肯定其是恶化因素之一。
[Abstract]:Objective To observe the effect of glycosylated low density lipoprotein (gly-LDL) on human vascular endothelial cell growth and antioxidant related factors, to explore the correlation between diabetic patients with vascular endothelial injury and gly-LDL and the possible mechanism. The medium and different concentration method (group G1, 0.06 mg/L, 0.12 mg/L G2 group, G3 group, 0.24 mg/L gly-LDL) human umbilical vein endothelial cells (HUVECs) of 24 h, 48 h and 72 h, the control group (Ctr), the supernatant was detected SOD and MDA levels, and impact on the migration of vascular endothelial cells by cell scratch assay to observe the gly-LDL, using semi quantitative real-time PCR (realtime quantitative PCR, RT-q PCR) expression methods for the determination of Mn-SOD and E NOS mRNA. Results compared with the control group transfer function of different concentrations of gly-LDL could inhibit vascular endothelial cells. The effect of gly-LDL is low or high concentration in human umbilical vein endothelial cells, With the increase of the time the content of MDA and activity of SOD is increased; the role of gly-LDL in the middle concentration of human umbilical vein endothelial cells, with the increase of time the activity of SOD was decreased, MDA content was first increased and then decreased. The expression level of mRNA Mn-SOD were 48 h and 72 h were down, e NOS the expression level of 24 h and 48 h were downregulated, 72 h up-regulated. Conclusion Gly-LDL and vascular complications in patients with diabetes may have a certain relationship, but not sure it is one of the factors of deterioration.

【作者单位】：同济大学附属同济医院内分泌科;复旦大学附属金山医院营养科;上海中医药大学附属普陀医院内分泌科;
【基金】：国家“863”高技术研究发展计划(2014AA022304)
【分类号】：R587.2;R54
【正文快照】： (MDA)levels in the cell culture supernatant were detected.The migrate function of endothelial cellsw as assessed by scratch test.M n-SOD and e NOS mRNA expressions w ere determined by real-timequantitative PCR(RT-q PCR).Results Gly-LDL at different conce

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