接种丛枝菌根真菌对小麦根内氮磷转运蛋白基因表达的影响

发布时间：2018-01-04 02:36

本文关键词：接种丛枝菌根真菌对小麦根内氮磷转运蛋白基因表达的影响　出处：《西北农林科技大学》2016年硕士论文　论文类型：学位论文

【摘要】：研究小麦-丛枝菌根(AM)真菌共生体内氮磷转运蛋白基因表达的调控机理对于深入了解此共生体系中氮磷养分的吸收转运机制具有重要意义。本试验研究了接种4个不同种类的AM真菌(A.delicata、C.etunicatum、F.mosseae和R.intraradices)对小麦菌根内不同类型氮磷转运蛋白基因表达的调控;在此基础上,通过设置遮阴处理和不同接种量处理研究了碳分配信号在调控小麦菌根内氮磷转运蛋白基因表达过程中的作用,并且在小麦中尝试克隆了AM真菌诱导表达的新铵态氮转运蛋白(AMT)基因,论文主要研究结果如下:1.接种的四种丛枝菌根真菌中只有C.etunicatum显著提高小麦地上部生物量,总吸磷量和总吸氮量。2.接种AM真菌局部下调了小麦根内受磷饥饿诱导的磷转运蛋白基因(TaPHT1.2和TaPT4)的表达,而局部上调了受AM真菌特异诱导的磷转运蛋白基因(TRIae;Pht1;10和TRIae;Pht1;11)的表达。接种AM真菌对小麦根内氮转运蛋白基因表达的影响比较复杂,主要包括局部下调、系统下调或者没有影响,且因不同菌种和不同基因型而异。3.小麦菌根侵染率随着接种量的增加而提高,与对照相比,接种显著提高了小麦地上部生物量、氮磷养分吸收量、光合能力、光合产物的累积量;最大接种量(200g)处理小麦各生长参数均显著高于最小接种量处理(20g);遮荫显著降低了小麦各生长指标;与对照相比,接种显著下调了磷转运蛋白(PHT1.2和PT4)和氮转运蛋白(NRT1.1、NRT1.2、NRT2.1、NRT2.2和AMT1.2)的表达,但不同接种量之间没有显著性差异,遮阴处理对氮磷转运蛋白基因表达没有影响。4.新克隆的铵态氮转运蛋白(AMT)基因(D-AMT)的蛋白结构符合AMT基因的特点,而且与节节麦(Aegilops tauschii)、二穗短柄草(Brachypodium distachyon)和水稻中AMT基因高度同源,,基因定量结果表明D-AMT基因主要在小麦根中受AM真菌诱导表达,而在叶片中的表达量较低,在茎中几乎不表达。荧光原位杂交(FISH)分析表明,D-AMT基因在小麦根系细胞膜上表达。
[Abstract]:Study on AMM of wheat-arbuscular mycorrhizae. The regulation mechanism of nitrogen-phosphorus transporter gene expression in symbiotic fungi is of great significance in understanding the absorption and transport mechanism of nitrogen and phosphorus nutrients in the symbiotic system. This experiment was conducted to study the inoculation of four different kinds of AM fungi. (. A.delicata. C. etunicatum F. mosseae and R. intraradices) regulated the expression of different types of nitrogen and phosphorus transporter genes in wheat mycorrhizae. On this basis, the role of carbon allocation signal in regulating the expression of nitrogen and phosphorus transporter gene in wheat mycorrhizal was studied by shading and inoculation. The new ammonium nitrogen transporter (AMT) gene induced by AM fungi was cloned in wheat. The main results were as follows: 1. Of the four mycorrhizal fungi inoculated, only C.etunicatum significantly increased the aboveground biomass of wheat. Total phosphorus uptake and total nitrogen uptake. Inoculation with AM fungi partially down-regulated the expression of phosphorous transporter genes TaPHT1.2 and TaPT4 induced by phosphorus starvation in wheat roots. The phosphorous transporter gene (TRIaeae), which was specifically induced by AM fungi, was locally up-regulated. Pht1; 10 and TRIaeae; Pht1; The effects of AM fungi inoculation on the expression of nitrogen transporter gene in wheat root were complicated, including local down-regulation, systemic down-regulation or no effect. The mycorrhizal infection rate of wheat increased with the increase of inoculation amount. Compared with the control, inoculation significantly increased the aboveground biomass and uptake of nitrogen and phosphorus. Photosynthetic capacity, accumulation of photosynthetic products; The growth parameters of wheat treated with maximum inoculation amount (200g) were significantly higher than that of the minimum inoculation treatment (20g / g). Shading significantly reduced the growth indexes of wheat. Compared with the control, the P transporter (PHT 1.2 and PT4) and the nitrogen transporter (NRT1.1 / NRT1.2 / NRT2.1) were significantly down-regulated by inoculation. NRT2.2 and AMT 1.2) expression, but there was no significant difference between different inoculation amount. Shading treatment had no effect on the expression of N-P transporter gene .4.The protein structure of the newly cloned ammonium nitrogen transporter gene (D-AMT) was consistent with that of AMT gene. It was also highly homologous to the AMT gene of Aegilops tauschii and Brachypodium distachyon. The results of quantitative analysis showed that D-AMT gene was mainly induced by AM fungi in wheat roots, but low in leaves. Fluorescence in situ hybridization (fish) analysis showed that the D-AMT gene was expressed on the cell membrane of wheat root system.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S512.1

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