不同环境因子对海洋细菌Pseudoalteromonas issachenkoniiHZ引导糖基转移酶基因表达的影响

发布时间：2018-01-04 23:30

  本文关键词：不同环境因子对海洋细菌Pseudoalteromonas issachenkoniiHZ引导糖基转移酶基因表达的影响　出处：《食品工业科技》2016年22期 　论文类型：期刊论文

  更多相关文章： 海洋细菌Pseudoalteromonas issachenkonii HZ 引导糖基转移酶 实时荧光定量PCR

【摘要】：为了研究海洋细菌Pseudoalteromonas issachenkonii HZ所产多糖的生物合成基因簇,首先需要克隆到在多糖合成中起关键作用的引导糖基转移酶基因。通过设计引物,采用PCR技术从海洋细菌Pseudoalteromonas issachenkonii HZ成功克隆到该基因,并通过实时荧光定量PCR技术检测了温度、p H及海盐浓度对引导糖基转移酶基因表达量的影响。结果表明:低温有利于引导糖基转移酶的表达,随着温度的上升,引导糖基转移酶基因的表达量先增后减,在20 h时,引导糖基转移酶的表达量急剧上升,15℃和35℃引导糖基转移酶表达量分别是20℃表达量的5.2倍、5.8倍;海盐浓度为4.5%时引导糖基转移酶的表达量为海盐浓度3.5%的2.5倍,海盐浓度为5.5%时引导糖基转移酶的表达量为海盐浓度3.5%的2.0倍;10 h时p H为8、9的培养基中引导糖基转移酶的表达量分别为p H为7的1.64和1.67倍。该结果为探究菌体环境适应性机制提供了一定的基础。
[Abstract]:In order to study the biosynthesis gene cluster of polysaccharides produced by marine bacteria Pseudoalteromonas issachenkonii HZ. First of all, we need to clone the leading glycosyltransferase gene which plays a key role in the synthesis of polysaccharides. The gene was cloned successfully from marine bacteria Pseudoalteromonas issachenkonii HZ by PCR. The effects of temperature pH and sea salt concentration on the gene expression of guided glycosyltransferase were detected by real-time fluorescence quantitative PCR. The results showed that low temperature was beneficial to the expression of glycosyltransferase. With the increase of temperature, the expression of leading glycosyltransferase gene increased first and then decreased. At 20 h, the expression of guided glycosyltransferase increased sharply. The expression of glycosyltransferase at 15 鈩，

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