外源褪黑激素促进绒山羊皮肤毛囊相关基因表达差异的研究

发布时间：2018-01-06 09:27

本文关键词：外源褪黑激素促进绒山羊皮肤毛囊相关基因表达差异的研究　出处：《畜牧兽医学报》2016年11期 　论文类型：期刊论文

【摘要】：旨在通过对非长绒期阿尔巴斯型绒山羊进行褪黑激素埋植,研究褪黑激素对羊绒周期性生长的调控,以期借此延长绒山羊毛囊的兴盛期,提高羊绒产量。将试验羊随机分成两组:埋植褪黑激素的试验组(T)和对照组(C),每组3只(试验组(TI、T2和T3)和对照组(C1、C2和C3)),采集绒山羊的皮肤组织进行组织切片,染色观察埋植褪黑激素对毛囊生长诱导作用。分别抽提两组样本总RNA,逆转录合成相应被标记的cDNA探针,采用Agilent绵羊的8×15K规格全基因组表达谱芯片进行杂交,筛选差异表达基因。利用实时荧光定量PCR技术进行验证。结果表明,从组织学分析可观察到埋植褪黑激素对毛囊生长具有明显的促进作用;芯片数据显示,筛选出差异表达的基因95个,其中61个表达上调、34个下调。GO分析基因数量分布情况:参与分子功能的基因占47.78%,参与生物学过程的基因占33.89%,组成细胞成分的基因占18.33%。与对照组的样本相比,埋植褪黑激素后相关基因的差异性表达涉及毛囊生长及周围皮肤附属物形态发生等生物学过程。这些差异性表达的基因为绒山羊毛囊生长及周期性调控的基因功能研究提供极有价值的参考。
[Abstract]:The aim of this study was to investigate the regulation of melatonin on the periodic growth of cashmere by embedding melatonin in non-long-period Albanian cashmere goats in order to prolong the prosperous period of cashmere goat hair follicles. To increase cashmere yield, the sheep were randomly divided into two groups: the experimental group implanted with melatonin, and the control group, with 3 rats in each group (Ti T 2 and T 3) and C 1 in the control group. The skin tissues of Cashmere goats were collected and sections were made to observe the induction effect of melatonin on the growth of hair follicles. The total RNA was extracted from two groups of samples. The corresponding labeled cDNA probe was synthesized by reverse transcription and hybridized with the whole genome expression microarray of Agilent sheep (8 脳 15K). The differentially expressed genes were screened and verified by real-time fluorescence quantitative PCR. The results showed that melatonin implantation could obviously promote the growth of hair follicles. Chip data showed that 95 differentially expressed genes were screened, 61 of which were up-regulated, 34 down-regulated .go analysis of the number of genes: 47.78% genes involved in molecular function. The genes involved in the biological process accounted for 33.89 and the genes that made up the cell components accounted for 18.33. Compared with the control group samples. The differential expression of genes related to melatonin implantation involves biological processes such as hair follicle growth and the morphogenesis of skin appendages around the hair follicles. These differentially expressed genes are genes of hair follicle growth and periodic regulation in cashmere goats. It can provide valuable reference for research.
【作者单位】：内蒙古自治区农牧业科学院;中国科学院昆明动物研究所;鄂托克前旗农牧业局;鄂托克前旗兽医局;鄂托克前旗北极神绒牧业研究所;
【基金】：内蒙古自治区自然科学基金项目(2014BS0333) 国家自然科学基金项目(31260545) 国家公益性行业(农业)科研专项(201303059) 内蒙古农牧业科学院青年创新基金项目(2013QNJJM05)
【分类号】：S827.2
【正文快照】： 内蒙古绒山羊是重要的绒用动物,羊绒被用来制造奢侈针织物,拥有很高的经济价值。绒山羊的毛被主要分为有髓的粗毛和无髓的绒毛两种类型,它们分别由初级毛囊和次级毛囊产生,它们在结构和组成上非常相似,而在细度上存在明显的差别,这种差别的产生是由相应毛囊的形态和尺寸决定的

【参考文献】