血浆MAL基因甲基化检测对于肺癌早期诊断的意义

发布时间：2018-01-07 08:02

本文关键词：血浆MAL基因甲基化检测对于肺癌早期诊断的意义　出处：《现代肿瘤医学》2016年13期 　论文类型：期刊论文

更多相关文章：肺癌 肺结核 血浆DNA MAL 甲基化 诊断

【摘要】：目的:研究肺癌患者、肺结核患者及健康人群T淋巴细胞相关蛋白(T-lymphocyte maturation-associated protein,MAL)基因启动子区的甲基化状态,进一步探讨血浆MAL基因甲基化检测对于肺癌早期诊断及鉴别肺癌与肺结核疾病的意义。方法:采用甲基化特异性PCR(methylation special PCR,MSP)方法,检测75例肺癌组织标本、癌旁组织标本和对应的血浆标本及58例肺结核组织标本及对应的血浆标本的MAL基因启动子区甲基化状态;同时检测30例正常人群的血浆标本MAL甲基化状态作为对照。结果:肺癌组织和癌旁组织中MAL基因甲基化发生率分别为78.7%(59/75)和2.7%(2/75),差别具有统计学意义(P0.001);肺癌和肺结核组织中MAL基因甲基化发生率分别为78.7%(59/75)和3.4%(2/58),差别具有统计学意义(P0.001);肺癌患者与肺结核患者对应的血浆中MAL基因甲基化发生率分别73.3%(55/75)和1.7%(1/58),差别也具有统计学意义(P0.001)。正常健康人群的血浆标本中未发现MAL基因甲基化改变;肺癌组和肺结核组分别与健康人群组比较,肺癌组明显高于健康人群(P0.001),而肺结核组患者与健康人群比较无明显差异(P=0.47)。组织标本和血浆标本的检出率具有一致性(P0.05)。根据病理类型、TNM分期、性别、吸烟史等对肺癌患者进行分组,各组之间均无明显差异(P0.05)。结论:MSP法检测血浆MAL基因甲基化状态,可以作为一种有潜力的肺癌早期诊断方法,对于鉴别肺癌和肺结核也有一定的帮助。
[Abstract]:Objective: To study the patients with lung cancer, T lymphocyte associated protein in patients with pulmonary tuberculosis and healthy people (T-lymphocyte maturation-associated, protein, MAL) gene promoter methylation status, to further explore the significance of detection of plasma MAL gene promoter methylation in lung cancer early diagnosis and identification of lung cancer and pulmonary tuberculosis disease. Methods: methylation specific PCR (methylation special PCR, MSP) detection method, 75 cases of lung cancer tissues, paracancerous MAL plasma samples of plasma samples and corresponding tissue samples and 58 cases of pulmonary tuberculosis tissue samples and corresponding with the methylation status of the promoter region; at the same time the plasma samples of MAL methylation status in 30 cases of normal people as control. Results: the methylation of MAL gene in lung cancer tissues and adjacent tissues of incidence were 78.7% (59/75) and 2.7% (2/75), the difference was statistically significant (P0.001); lung cancer and lung tissue. The MAL gene methylation rate was 78.7% (59/75) and 3.4% (2/58), the difference was statistically significant (P0.001); MAL gene methylation in patients with lung cancer and corresponding pulmonary tuberculosis patients in the incidence rate of plasma 73.3% (55/75) and 1.7% (1/58), the difference has statistical significance (P0.001). The change of MAL gene methylation was found in the plasma samples of normal healthy people; lung cancer group and pulmonary tuberculosis group compared with the healthy group, lung cancer group was significantly higher than that in healthy people (P0.001), and pulmonary tuberculosis patients and the healthy controls had no obvious difference (P=0.47). The detection rate of tissue and plasma samples with consistency (P0.05). According to the pathological type, TNM stage, sex, group of lung cancer patients with smoking history, between the groups were not significantly different (P0.05). Conclusion: the detection of plasma MAL gene promoter in MSP method, can be used as a potential early lung cancer The diagnosis is also helpful for the identification of lung cancer and tuberculosis.

【作者单位】：西安交通大学第二附属医院胸外科;西安市胸科医院外科;
【基金】：陕西省科技攻关资助项目(编号:2012k13-02-30)
【分类号】：R734.2
【正文快照】： Modern Oncology 2016,24(13):2055-2058【修回日期】2016-01-262西安市胸科医院外科,陕西西安710004甲基化状态,可以作为一种有潜力的肺癌早期诊断方法,对于鉴别肺癌和肺结核也有一定的帮助。肺癌是一种十分常见的呼吸道恶性肿瘤,在我国,其疾病发病率和死亡率在所有恶性肿瘤

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