番茄GRAS转录因子家族基因SIGLD1的克隆及VIGS载体构建

发布时间：2018-01-07 03:15

本文关键词：番茄GRAS转录因子家族基因SIGLD1的克隆及VIGS载体构建　出处：《基因组学与应用生物学》2016年08期 　论文类型：期刊论文

【摘要】：本研究以醋栗番茄(LA2184)为材料,运用生物信息学方法和PCR技术,预测SIGLD1基因与番茄抗冷的相关性,设计SIGLD1基因的特异性引物,克隆出片段长度为413 bp的序列。以番茄八氢番茄红素脱氢酶(phytoenedesaturase,PDS)基因作为阳性对照,该基因克隆片段长度为427 bp。系统进化树显示,SIGLD1基因与拟南芥中已发表过的抗冷基因聚类在一起,利用RT-PCR技术,检测SIGLD1基因在不同冷处理时间点上的表达量,发现SIGLD1基因在3~6 h的表达量最高,说明该基因在番茄的抗冷过程中发挥重要作用。本研究成功构建出SIGLD1、PDS基因的病毒诱导基因沉默的重组载体。构建的p TRV2-SIGLD1、p TRV2-PDS重组载体将为下一步研究番茄中SIGLD1基因的功能验证奠定实验基础。
[Abstract]:In this study, currant tomato (LA2184) as material, using bioinformatics methods and PCR technique, the correlation of SIGLD1 gene and prediction of tomato resistance to cold, the specific primers of SIGLD1 gene, cloned fragment length 413 BP sequence. In eight tomato phytoene dehydrogenase (phytoenedesaturase, PDS) gene as a positive the control, clone the gene length of 427 bp. phylogenetic tree showed that the SIGLD1 gene in Arabidopsis have been published in cold resistance gene cluster together, using RT-PCR technology, the detection of SIGLD1 gene in different time of the cold treatment on the expression of SIGLD1 gene was found in the highest, the expression of 3~6 h, indicating that the gene plays an important role in the process of cold resistance of tomato. This study successfully constructed SIGLD1 and PDS gene of virus induced gene silencing. The recombinant vector construction of P TRV2-SIGLD1, P TRV2-PDS recombinant vector will be the next step The experimental basis for the study of the functional verification of SIGLD1 gene in tomato was established.

【作者单位】：东北农业大学园艺学院;
【基金】：现代农业产业技术体系专项资金黑龙江省大宗蔬菜新品种选育“黑龙江省大宗蔬菜新品种选育”(GA15B103-1)项目资助
【分类号】：Q943.2
【正文快照】： Cloning and VIGS Vector Construction of GRAS Transcription FactorsFamily Gene SIGLD1 from TomatoNiu Yiling Jiang Xiuming Xu Xiangyang*College of Horticulture,Northeast Agricultural University,Harbin,150030*Corresponding author,xxy709@126.comGRAS转录因子

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