夏眠期仿刺参肠组织实时荧光定量PCR分析中内参基因的筛选
发布时间：2018-01-09 09:30
本文关键词：夏眠期仿刺参肠组织实时荧光定量PCR分析中内参基因的筛选 出处：《中国海洋大学学报(自然科学版)》2016年07期 论文类型：期刊论文
【摘要】：在仿刺参(Apostichopus japonicus)正常时期、夏眠初期和中期,应用实时荧光定量PCR技术,检测CA043、dpolm、ro60、grb2、hiat1、nlrc4、farp1、cyc和gapdh9个候选内参基因在肠组织中的表达稳定性。利用DeltaCt、GeNorm、NormFinder和BestKeeper 4种程序进行统计分析。发现上述条件中,9个候选内参基因在上述条件中的表达稳定性存在一定差异,不同的软件处理分析,得到的基因稳定性排序不完全一致。综合4种程序的方法,筛选出基因grb2表达最稳定,可用作内参基因,其次为基因ro60及dpolm,而表达最不稳定的是CA043,不适宜作为内参基因。本研究为仿刺参中基因表达定量分析奠定了基础,同时为仿刺参中内参基因的选择提供一定参考依据。
[Abstract]:The sea cucumber (Apostichopus japonicus) in normal times, the early and mid-term, real-time fluorescence quantitative PCR technology, application of detection of CA043, dpolm, ro60, Grb2, hiat1, nlrc4, farp1, CYC and gapdh9 expression stability of candidate reference genes in intestinal tissue. By using DeltaCt, GeNorm, NormFinder and BestKeeper 4 program the statistical analysis found. These conditions, there are some differences in the expression stability of 9 candidate reference genes in the above conditions, analysis and treatment of different sort, gene of the stability is not entirely consistent. Methods 4 procedures, screening out the expression of the Grb2 gene in the most stable, can be used as a reference gene, followed by gene ro60 and dpolm, and the expression of the most unstable CA043 is not suitable as a reference gene. This study laid the foundation for the quantitative analysis of gene expression of Apostichopus japonicus, while providing generic thorn ginseng reference gene selection A certain reference.
【作者单位】： 大连海洋大学农业部北方海水增养殖重点实验室;中V锖Ｑ蟠笱ШＱ笊镆糯в胗纸逃恐氐闶笛槭，
本文编号：1400874
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