C14族R2R3-MYB基因调控杨树抗锈菌过敏性反应

发布时间：2018-01-09 15:25

  本文关键词：C14族R2R3-MYB基因调控杨树抗锈菌过敏性反应　出处：《北京林业大学学报》2016年07期 　论文类型：期刊论文

  更多相关文章： 转录因子 转录调控 美洲黑杨×毛果杨 欧美杨 落叶松-杨栅锈菌

【摘要】：由落叶松-杨栅锈菌引起的杨树叶锈病分布广泛,危害严重。为探究杨树C14族R2R3-MYB基因在杨树抗锈菌过敏性反应中的调控作用,对接种了落叶松-杨栅锈菌E4强致病性生理小种的2种杨树叶片进行症状观察、感病指数判定并对2种杨树C14族共6条R2R3-MYB基因表达量变化情况进行分析。接种7 dpi后,弱感病性树种杂交杨叶片产生的夏孢子堆数量显著少于感病树种欧美杨。杂交杨叶片4 dpi开始出现过敏性反应症状,欧美杨未观察到类似症状。表明过敏性反应可有效阻止锈菌夏孢子堆形成,降低杨树感病性。杂交杨与欧美杨R2R3-MYB基因表达量变化趋势相反,预测杨树C14族R2R3-MYB基因为杨树与病原互作过程中调控过敏性反应的正向调控因子。本研究为进一步系统研究杨树R2R3-MYB基因与过敏性反应的调控机理及杨树抗病育种工作提供理论依据。
[Abstract]:Poplar leaf rust caused by Larix gmelinifera was widely distributed and seriously damaged. In order to investigate the role of R2R3-MYB gene in the allergic response of poplar to rust resistance, R2R3-MYB gene of poplar C14 was studied. The leaf symptoms of two species of poplar inoculated with AE4 strong pathogenicity of Larix gmelinifera were observed. Susceptibility index was used to determine and analyze the expression of 6 R2R3-MYB genes in two species of poplar C14. After inoculation for 7 dpi, the expression of R2R3-MYB gene was analyzed. The number of summer spores produced in poplar leaves of weakly susceptible poplar was significantly less than that of susceptible poplar Euramerican poplar. The anaphylaxis symptoms began to appear in the leaves of hybrid poplar for 4 dpi. No similar symptoms were observed in Euramerican poplar, which indicated that anaphylaxis reaction could effectively prevent the formation of summer spores and reduce the susceptibility of poplar. The change trend of R2R3-MYB gene expression in hybrid poplar and Euramerican poplar was opposite. It is predicted that the R2R3-MYB gene of poplar C14 is a positive regulator of allergic reaction in poplar interaction with pathogen. This study is intended to further systematically study the relationship between R2R3-MYB gene and allergies in poplar. The regulation mechanism of sex response and the breeding of poplar disease resistance provide theoretical basis.
【作者单位】： 东北林业大学林学院;
【基金】：国家自然科学基金项目(31500524) 中央高校基本科研业务费专项(2572015EY02,2572015CA09,2572016CA04) 黑龙江省博士后基金(LBH-Z11248) 高等学校博士学科点专项科研基金(博士点新教师基金20100062120002)
【分类号】：S763.7
【正文快照】： 专性寄生真菌落叶松-杨栅锈菌(Melampsoralarici-populina)主要侵染青杨派(SectionTacamahaca)、黑杨派(Section Aigeiros)及其杂交种叶片,引起杨树叶锈病[1]。落叶松-杨栅锈菌完整生活史共产生性孢子、锈孢子、夏孢子、冬孢子和担孢子5种孢子,其中无性阶段的夏孢子在生长季反，

本文编号：1401960