毛竹TIPs基因家族成员组织表达模式研究

发布时间：2018-01-09 15:26

本文关键词：毛竹TIPs基因家族成员组织表达模式研究　出处：《林业科学研究》2016年04期 　论文类型：期刊论文

【摘要】：[目的]通过研究毛竹TIPs的分子特征和表达模式,为揭示逆境胁迫条件下TIPs在竹子中的作用提供证据,为培育抗逆的植物新品种提供新的基因资源。[方法]以毛竹为对象,利用生物信息学方法对毛竹基因组中的TIPs基因进行了全面分析,采用实时荧光定量PCR技术分析基因在不同组织及干旱、水淹和Na Cl胁迫下的表达模式。[结果]毛竹基因组中含有6个TIPs同源基因,分别隶属于3个亚类(TIP1、TIP2和TIP4)。基因结构预测显示,Pe TIP1;1、Pe TIP1;2、Pe TIP2;2和Pe TIP4;2由2个外显子和1个内含子组成,而Pe TIP2;1和Pe TIP4;1由3个外显子和2个内含子组成。蛋白结构分析显示,毛竹6个TIPs均具有2个典型的NPA结构域和4个ar/R模体。通过转录组数据分析基因的组织表达特异性,结果表明Pe TIP1;1在各组织中的表达丰度均较高;Pe TIP1;2主要在花中表达;Pe TIP2;1在根和鞭中表达量较高;Pe TIP2;2在根中特异表达;Pe TIP4;1在叶片中表达丰度最高;Pe TIP4;2在笋和鞭中表达水平较高,在根中最低。实时定量PCR结果分析证明,干旱处理后毛竹根中Pe TIP4;1的表达量显著升高(p0.01),Pe TIP2;1、Pe TIP2;2和Pe TIP4;2表达受到抑制(p0.01);水淹处理后根中Pe TIP1;1和Pe TIP4;1表达量显著增加(p0.01),Pe TIP1;2、Pe TIP2;2、和Pe TIP4;2则显著降低(p0.01);Na Cl处理后根中6个Pe TIPs的表达量均显著增加(p0.01)。干旱处理后毛竹叶片中Pe TIP1;1、Pe TIP1;2和Pe TIP4;1表达量均显著增加(p0.01);水淹处理后叶片中Pe TIPs表达量均显著提高(p0.01);Na Cl处理后叶片中Pe TIP2;1表达受到明显抑制(p0.01)。[结论]Pe TIPs可能在毛竹抵抗干旱、水淹和Na Cl等非生物胁迫中发挥着不同程度的作用。
[Abstract]:[Objective] to study the molecular characteristics and expression patterns of TIPs in Phyllostachys pubescens, to provide evidence for revealing the role of TIPs in bamboo under stress stress, and to provide new genetic resources for the cultivation of new stress resistant varieties of Phyllostachys pubescens (Phyllostachys pubescens). [Methods] the TIPs gene in the genome of Phyllostachys pubescens was comprehensively analyzed by bioinformatics, and real-time fluorescence quantitative PCR was used to analyze the gene in different tissues and drought. The expression pattern of water flooded and NaCl stress. [Results: there were 6 TIPs homologous genes in the genome of Phyllostachys pubescens, belonging to three subclasses, TIP1, TIP2 and TIP4, respectively. (1) Pe TIP1; 2Pe TIP2; 2 and Pe TIP4; 2consists of 2 exons and 1 intron, while Pe TIP2; 1 and Pe TIP4; 1 was composed of 3 exons and 2 introns. The 6 TIPs of Phyllostachys pubescens had two typical NPA domains and four ar/R motifs. The specific expression of Pe TIP1 was analyzed by transcriptional data. 1the expression abundance was higher in all tissues; Pe TIP1; (2) mainly expressed in flowers; Pe TIP2; 1High expression in root and whip; Pe TIP2; 2specific expression in roots; Pe TIP4; 1the highest abundance in leaves; Pe TIP4; 2the expression level of Pe TIP4 was higher in bamboo shoot and whip, but lowest in root. The results of real-time quantitative PCR showed that Pe TIP4 in bamboo root after drought treatment; (1) the expression of Pe TIP2 was significantly higher than that of P0. 01 and Pe TIP2; (1) Pe TIP2; 2 and Pe TIP4; 2the expression of P0. 01 was inhibited; Pe TIP1 in roots after waterflooded treatment; 1 and Pe TIP4; (1) the expression of Pe TIP1 increased significantly; 2Pe TIP2; 2, and Pe TIP4; (2) P 0.01 was significantly decreased; The expression of six Pe TIPs in roots increased significantly after NaCl treatment, and Pe TIP1 in leaves of Phyllostachys pubescens after drought treatment. (1) Pe TIP1; 2 and Pe TIP4; (1) the expression of p0.01 was significantly increased; The expression of Pe TIPs in leaves increased significantly after waterflooding treatment. Pe TIP2 in leaves treated with NaCl; 1 the expression of P0. 01 was significantly inhibited. [Conclusion Pe TIPs may play an important role in resistance of Phyllostachys pubescens to drought, flooding and NaCl stress.
【作者单位】：国际竹藤中心国家林业局竹藤科学与技术重点开放实验室;
【基金】：国家科技支撑计划课题“竹藤优异种质创制创新与种苗培育标准化示范”(2015BAD04B01) 国际竹藤中心基本科研业务费专项资金项目(1632015008)
【分类号】：S795.7
【正文快照】： 干旱、水淹和盐胁迫是影响植物生长、限制作物产量的主要因素。快速调节细胞渗透压和维持水分平衡是植物应对非生物胁迫的重要方式。维护细胞内渗透势平衡对保持细胞内稳态至关重要,这个过程需要大量的水通道蛋白(Aquaporins,AQPs)调节完成[1-2]。AQPs是微生物、植物和动物细

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