毛果杨HDA902基因的克

发布时间：2018-01-12 21:16

本文关键词：毛果杨HDA902基因的克隆、序列分析和亚细胞定位　出处：《分子植物育种》2016年05期 　论文类型：期刊论文

【摘要】：组蛋白去乙酰化酶(histone deacetylases,HDACs)是一类重要的酶,催化组蛋白去乙酰化,参与组蛋白乙酰化状态的调节,对染色体结构修饰和基因表达调控发挥着重要的作用。为了研究毛果杨HDA902基因的亚细胞定位,进而为研究其功能奠定基础,采用PCR的方法克隆了毛果杨组蛋白去乙酰化酶基因HDA902的编码序列,采用生物信息学方法分析了该基因所编码的蛋白质序列,并构建了HDA902-GFP融合基因表达载体,通过其在洋葱表皮细胞的表达来确定HDA902蛋白的亚细胞定位。研究结果表明,毛果杨HDA902基因的开放阅读框为1 500 bp,编码1个由499个氨基酸残基组成的蛋白质。序列比对和结构域分析显示,毛果杨HDA902蛋白与其他植物同源蛋白具有一个保守的结构域HDAC3。系统进化树分析表明,毛果杨HDA902蛋白在进化上与桃(Prunus persica)HDAC(XP_007200978.1)和苹果(Malus domestica)HDA19-like(XP_008348955.1)的亲缘关系较近。启动子分析表明,毛果杨HDA902基因的启动子序列包含5UTR Pyrich stretch、ARE、ACE和HSE等多个与逆境和光响应相关的顺式作用元件。亚细胞定位分析表明,毛果杨HDA902蛋白主要存在于细胞质中。这些研究结果为进一步研究毛果杨组蛋白去乙酰化酶HDA902基因的功能提供了理论基础。
[Abstract]:Histone deacetylase (histone deacetylases HDACs) is a kind of important enzyme catalyzed histone acetylation involved in histone acetylation state regulation, plays an important role in chromosome structure modification and gene expression regulation. In order to study the subcellular localization of HDA902 gene of Populus trichocarpa, and lay the foundation in order to study the function of encoding sequence cloned p.trichocarpa histone deacetylase gene HDA902 by PCR method, using bioinformatics analysis of the gene encoding the protein sequence, and constructed the HDA902-GFP fusion gene expression vector and its expression in the onion epidermal cells to determine the subcellular localization of HDA902 protein. The results showed that p.trichocarpa HDA902 gene ORF is 1500 BP, encoding 1 consisting of a protein of 499 amino acids. Sequence alignment and domain analysis showed that, Populus trichocarpa HDA902 protein has a conserved domain HDAC3. phylogenetic tree analysis indicated that the homology with other plants, Populus trichocarpa HDA902 protein in evolution and peach (Prunus persica) HDAC (XP_007200978.1) and apple (Malus domestica) HDA19-like (XP_008348955.1) close genetic relationship. The promoter analysis showed that start sequence of HDA902 gene of Populus trichocarpa contains 5UTR Pyrich stretch, ARE, ACE and HSE and other adversities and light responsive cis elements related. Subcellular localization analysis showed that p.trichocarpa HDA902 protein in the cytoplasm. These results for the further study of p.trichocarpa histone deacetylase the function of HDA902 gene provides a theoretical basis.

【作者单位】：东北林业大学林木遗传育种国家重点实验室;
【基金】：黑龙江省博士后基金项目(LBH-Z13006) 国家自然科学基金青年科学基金项目(31200497)共同资助
【分类号】：S792.11
【正文快照】： *通讯作者,maxj@nefu.edu.cnCloning,Sequence Analysis and Subcellular Localization of HDA902 Genefrom Populus trichocarpaLiu Chao Li Kailong Liu Chunjuan Ma Xujun*State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,15004

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