TaqMan-MGB探针实时PCR用于Duffy血型基因分型的研究

发布时间：2018-01-12 23:19

本文关键词：TaqMan-MGB探针实时PCR用于Duffy血型基因分型的研究　出处：《中国输血杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的应用Taq Man-MGB探针实时PCR技术,建立1个Duffy血型基因分型的新方法,了解大连地区汉族人群Duffy血型等位基因频率分布特征。方法设计并合成Taq Man-MGB探针实时PCR的引物和探针,应用TaqMan-MGB探针实时PCR对120例健康献血者的Duffy血型进行基因分型,对其重复性和最低检出限进行评价。并将该法和传统的等位基因特异性引物PCR(PCR-ASP)方法进行比较。结果 Taq Man-MGB探针实时PCR方法和PCR-ASP法对Duffy血型的基因分型结果是完全一致的。Taq Man-MGB探针实时PCR再次分型结果和初次基因分型的结果是完全一致的。Taq Man-MGB探针实时PCR的最低检测限是100 pg。大连汉族人群Duffy血型FY*A基因频率为93.3%,FY*B的基因频率为6.7%,其基因分布特点和国内其他地区汉族人群相似,无显著性差异(P0.05),但与浙江畲族相比,有显著性差异(P0.05)。Fya和Fyb抗原不配合几率为0.116 7。结论应用Taq ManMGB探针实时PCR技术建立的Duffy血型基因分型方法具有快速、简单、准确、灵敏、重复性好、通量高等特点,优于传统的PCR-ASP法。
[Abstract]:Objective to study the application of Taq Man-MGB real-time PCR technology, to establish a new method for 1 Duffy genotyping, understand the Han population in Dalian area Duffy blood type gene frequency distribution method. Primers and probes were designed and synthesized Taq Man-MGB probe real-time PCR, application of TaqMan-MGB probe real-time PCR in 120 healthy blood donors Duffy blood group genotyping, the repeatability and the lowest detection limit was evaluated. And the traditional method of allele specific primer PCR (PCR-ASP) methods are compared. Results Taq gene Man-MGB probe real-time PCR method and PCR-ASP method for Duffy blood typing results.Taq Man-MGB probe real-time PCR completely consistent again, the results of genotyping and initial genotyping is the lowest detection limit of.Taq Man-MGB probe real-time PCR identical is 100 PG. Duffy in Dalian Han population FY*A blood group gene frequency was 93. 3%, the gene frequency of FY*B was 6.7%, the gene distribution characteristics of Han population and other parts of the country are similar, no significant difference (P0.05), but compared with Zhejiang Yu, there was significant difference (P0.05).Fya and Fyb antigen with a probability of 0.116 7.. Conclusion Taq was used to establish the ManMGB probe real-time PCR Duffy blood group genotyping method is rapid, simple, accurate, sensitive, reproducible, high flux, PCR-ASP is superior to the traditional method.

【作者单位】：大连市血液中心;大连医科大学生物学教研室;
【分类号】：R440
【正文快照】： Duffy血型基因包含4个由单核苷酸多态性(SNP)产生的等位基因,分别为FY*A、FY*B、FY*X和FY*Fy[1]。共有5种表型:Fy(a+b-)、Fy(a-b+)、Fy(a+b+)、Fy(a-b+w)和Fy(a-b-)[2]。Duffy血型基因FY*A和FY*B,分别编码血型抗原Fya和Fyb[3]。FY*X导致Fyb抗原氨基酸的改变,使红细胞表面Fyb抗

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