AtPAP1基因在烟草中的表达

发布时间：2018-01-13 19:02

本文关键词：AtPAP1基因在烟草中的表达　出处：《烟草科技》2016年07期 　论文类型：期刊论文

【摘要】：AtPAP1(Arabidopsis thaliana production of anthocyanin pigment 1)是拟南芥花青素生物合成途径中的关键调控基因。为实现花青素在烟草中的表达,利用烟草腺毛特异表达启动子pro CYP71D16,构建At PAP1特异表达载体p CAMBIA1391-CYP71D16-PAP1,并将其与载体p CAMBIA1391-35S-PAP1通过农杆菌介导法进行烟草遗传转化,对所得的转基因烟株进行分子检测和表型观察,同时测定转基因烟叶中花青素的含量。结果表明:35S::At PAP1烟株叶片表面呈红色,腺头呈绿色,腺柄细胞无色;CYP71D16::At PAP1烟株叶片表面呈绿色,叶片分泌型腺毛的腺头呈明显粉红色;对照烟草叶片及叶面腺毛均呈绿色。HPLC检测结果表明,在35S::At PAP1转基因烟株中发现有矢车菊色素和天竺葵色素的积累;在CYP71D16::AtPAP1转基因烟株中仅发现矢车菊色素有少量积累;对照烟株中没有检测到花青素。本研究中实现了At PAP1基因在烟草中的表达,为研究烟草物质代谢提供了理论依据。
[Abstract]:AtPAP1 (Arabidopsis thaliana production of anthocyanin pigment 1) is a key regulatory gene in Arabidopsis anthocyanin biosynthesis. In order to realize the expression of anthocyanin in tobacco, expression promoter Pro CYP71D16 using At PAP1 to construct tobacco trichome specific, specific expression vector p CAMBIA1391-CYP71D16-PAP1, and with the vector p CAMBIA1391-35S-PAP1 by Agrobacterium mediated method of Tobacco Genetic Transformation, the transgenic tobacco plants by molecular detection and morphological observation, determination of anthocyanins in transgenic tobacco. The results showed that: 35S: At: PAP1 tobacco leaf surface is red, glandular head is green, adenophore CYP71D16: At PAP1: colorless cells; tobacco leaf surface is green, glandular head leaves secretory glandular hairs were pink; the control of tobacco leaves were green and glandular trichome.HPLC results showed that in the 35S:: At transgenic PAP1 Because of cyanidin and pelargonidin accumulation in tobacco plant; in the CYP71D16:: AtPAP1 transgenic tobacco plants that accumulate cyanidin; no tobacco control was detected in anthocyanin. In this study the expression of At PAP1 gene in tobacco, and provides a theoretical basis for the research of tobacco metabolism.

【作者单位】：河南农业大学烟草学院国家烟草栽培生理生化基地;
【基金】：中国烟草总公司基因组重大专项“烟草腺毛功能基因挖掘与分子调控研究”(110201401003 JY-03)
【分类号】：S572
【正文快照】： 花青素是一类广泛存在于植物体中的水溶性天然色素,属于黄酮类化合物,是目前发现的最有效的抗氧化剂之一,可以减少体内自由基的产生,在防癌抗癌方面具有重要作用[1-2]。通过食用含有花青素的食物可以增强身体免疫力,因此花青素还具有很高的营养价值和保健作用[3-4]。研究发现,

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