基于核基因序列JRD5680的核桃群体遗传多样性和遗传结构研究

发布时间：2018-01-14 00:00

本文关键词：基于核基因序列JRD5680的核桃群体遗传多样性和遗传结构研究　出处：《植物研究》2016年02期 　论文类型：期刊论文

【摘要】：以40个核桃(Juglans regia)地理群体及5个外类群植物为材料,利用苯丙氨酸解氨酶基因片段JRD5680序列对265个个体研究了我国不同地区核桃的群体遗传多样性、群体遗传结构以及单倍型地理分布。结果表明:核基因JRD5680序列长度为809 bp,其中G+C碱基含量为46.2%,74个简约性信息位点。共30个单倍型,单倍型多样性变异小,表现出较低的遗传多样性(H_d=0.370,π=0.005 3)。核桃群体间有明显的谱系地理学结构(N_(ST)G_(ST)),呈现地域性分布格局的特点。空间遗传结构分析表明,地理隔离距离与遗传距离之间也显示两者之间相关性显著(r=0.263 2;P=0.032 4*),不同地理分布核桃群体间存在着明显地理隔离效应。综合失配分析及中性检验的结果,可推断出近期历史上不同地区核桃没有发生群体扩张事件。遗传结构显示,核桃群体可分为两组(西南地区与其他地区)。AMOVA分析结果表明,核桃群体遗传变异主要存于群体之间(56.54%),不同种之间的遗传分化水平较高(F_(ST)=0.885)。单倍型系统发育树中核桃与外类群物种均能形成单系分支,说明JRD5680序列在胡桃科中的具有较高的分子鉴别率。
[Abstract]:In 40 walnut (Juglans regia) populations and 5 outgroup plants as the material, the use of phenylalanine in 265 individuals of Walnut in different regions of China population genetic diversity ammonialyase gene JRD5680 sequence, population genetic structure and geographical distribution of haplotypes. The results show that the nuclear gene JRD5680 sequence length is 809 BP, the G+C base content is 46.2%, 74 parsimony informative sites. A total of 30 haplotypes and haplotype diversity variation, showed low genetic diversity (H_d=0.370, P =0.005 3). Walnut populations of pedigree geography structure obviously (N_ (ST) G_ (ST)). Has the characteristics of geographical distribution pattern. The analysis shows that the spatial genetic structure, geographic distance and genetic distance between isolates also showed significant correlation between them (r=0.263 2; P=0.032 4*), different geographical distribution of walnut populations have obvious geographical isolation effect Comprehensive mismatch analysis and neutral test results can be inferred from the recent history of Walnut in different areas of population expansion events did not happen. The genetic structure showed that walnut populations could be divided into two groups (the southwest and other regions).AMOVA analysis showed that the genetic variation mainly exists in walnut between groups (56.54%), different kinds of between the high level of genetic differentiation (F_ (ST) =0.885). The haplotype phylogeny of walnut and outgroup species in the tree are able to form a monophyletic clade, JRD5680 sequence has high molecular identification in Juglandaceae in rate.

【作者单位】：西北大学生命科学学院;
【基金】：国家自然科学基金项目(31200500;41471038) 陕西省教育厅重点实验室科研计划(13JS094)
【分类号】：S664.1
【正文快照】： 植物群体遗传变异是进化的基础,这些变化的速率与现有的遗传多样性的数量成比例[1~2]。遗传多样性水平可以反映植物自身的生物学特征及适应自然环境变化和人为干扰的能力[3]。遗传结构是物种在空间上不同群体间和同一群体内不同个体间的遗传变异[4],群体遗传结构差异也是衡量

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