转BADH基因玉米自交系的抗旱耐盐性分析

发布时间：2018-01-14 14:38

本文关键词：转BADH基因玉米自交系的抗旱耐盐性分析　出处：《吉林农业大学学报》2016年03期 　论文类型：期刊论文

【摘要】：试验以T_4代转BADH基因玉米自交系(受体亲本为"丹988")为基础材料,逐年加代纯化,通过PCR检测、Southern blotting以及实时荧光定量PCR法(Quantitative real-time PCR,qRT-PCR)来验证BADH基因在T_5和T_6代株系中能否稳定的遗传及表达,同时对T_6代转化株系进行抗旱耐盐性鉴定,对T_5与T_6代转基因玉米各株系的农艺性状进行了调查分析。结果表明:外源BADH基因以单拷贝的形式整合到玉米基因组中,且BADH基因在转基因植株各组织中均有表达,其中在叶中的表达量最高,达到1.1,而在根中的表达量只有0.1;在不同株系间的表达量也不同。对T_6代阳性植株进行干旱与盐胁迫处理后,测定脯氨酸、POD活性等生理生化指标,结果表明都明显高于受体亲本;T_5与T_6代转基因株系在株高、茎粗、穗长等农艺性状上与受体亲本无差异。该试验获得了与对照受体亲本"丹988"相比抗旱耐盐性较强、且农艺性状无显著差异的转基因玉米自交系。
[Abstract]:T4 generation transgenic maize inbred lines with BADH gene (the recipient parent was "Dan 988") were used as the basic material, then purified year by year, and detected by PCR. Southern blotting and quantitative real-time PCR with real-time fluorescence. QRT-PCR was used to verify the stable inheritance and expression of BADH gene in T _ (5) and T _ s _ 6 lines, and to identify the resistance to drought and salt in the transformed lines of T _ (th) _ 6 generation. The agronomic characters of transgenic maize lines of T _ (5) and T _ (6) generation were investigated and analyzed. The results showed that the exogenous BADH gene was integrated into the maize genome in the form of a single copy. BADH gene was expressed in all tissues of transgenic plants, and the highest expression was found in leaves (1.1%), but only 0.1 in roots. After drought and salt stress, the activity of proline POD and other physiological and biochemical indexes were measured. The results showed that the expression of proline POD was significantly higher than that of recipient parents. There was no difference between T _ 5 and T _ 6 transgenic lines in agronomic characters such as plant height, stem diameter, ear length and other agronomic characters. This experiment obtained a stronger drought and salt tolerance than the control recipient parent "Dan 988". And no significant difference in agronomic traits of transgenic maize inbred lines.
【作者单位】：吉林农业大学农学院;
【基金】：国家转基因生物新品种培育科技重大专项(2011ZX08004-004)
【分类号】：S513
【正文快照】： 玉米(Zea mays L.)是世界上重要的粮食作物,也是重要的饲料和工业原料,在国民经济中占有重要的地位。随着全球气候变暖趋势加剧,干旱和土壤盐碱化成为影响玉米产量最主要的非生物胁迫因子[1]。近年来,随着生物技术的飞速发展,利用植物基因工程技术培育抗逆转基因玉米已经成为

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