呼吸道铜绿假单胞菌感染病灶藻酸盐合成相关基因的表达

发布时间：2018-01-15 04:29

本文关键词：呼吸道铜绿假单胞菌感染病灶藻酸盐合成相关基因的表达　出处：《陕西医学杂志》2016年12期 　论文类型：期刊论文

【摘要】：目的:获得一种呼吸道铜绿假单胞菌生物膜检测方法,探讨藻酸盐合成相关基因的表达对临床治疗的影响。方法:提取60例呼吸道铜绿假单胞菌感染患者气管内病变组织,实时荧光定量PCR检测藻酸盐合成相关基因algD、algB、algU、mucA的表达,60例患者根据是否耐药分组,分为耐药组(R组)40例,非耐药组(NR组)20例,根据治疗的难易程度,将60例患者分为治疗困难组(D组)38例,治疗容易组(E组)22例。并进行基因algD、algB、algU、mucA的表达与临床抗生素耐药及治疗难易之间的相关性分析。结果:经细菌培养证实的铜绿假单胞菌感染病例60例,呼吸道病变组织均有algD、algB、algU、mucA基因的表达;耐药组(R组)和非耐药组(NR组)对比,algD、algU表达有统计学差异,algB、mucA表达无统计学差异;容易组(E组)和困难组(D组)对比,algD、algU、algB、mucA差异无统计学意义;细菌耐药和治疗难易的相关性分析显示,细菌耐药和临床治疗难易相关。结论:algD与铜绿假单胞菌藻酸盐的合成有着直接的正相关性,可以作为判断是否有黏液型铜绿假单胞菌生长及生物膜形成的依据,而algU与algD的表达有正相关性。
[Abstract]:Objective: to obtain a respiratory Pseudomonas aeruginosa biofilm detection method, to investigate the expression of the alginate biosynthetic genes influence on clinical treatment. Methods: the extracted lesion tissue of patients with trachea in 60 cases of respiratory tract infection of Pseudomonas aeruginosa, real time fluorescence quantitative PCR detection of alginate biosynthetic genes algD, algB, algU, mucA the expression of 60 cases according to whether the resistance group, divided into resistance group (R group) 40 cases, non resistance group (NR group) 20 cases, according to the degree of difficulty of treatment, 60 patients were divided into treatment group (group D) difficulties in 38 cases, easy treatment group (E group) 22 cases. And algB, algD gene, algU, analysis of the correlation between the expression of mucA and clinical antibiotic resistance and treatment between difficulty. Results: the bacterial culture of Pseudomonas aeruginosa confirmed 60 cases of infection, respiratory tract lesions were algD, algB, algU, mucA gene expression; resistance group (group R) and non resistance The medicine group (NR group), algD, algU expression had significant difference, algB, mucA expression had no significant difference; easy group (E group) and difficult group (D group) algD, algU, algB, and mucA was no significant difference; correlation analysis of bacterial drug resistance and treatment difficulty showed that bacterial resistance and the clinical treatment difficult. Conclusion: the synthesis of algD and Pseudomonas aeruginosa alginate has positive correlation with direct, can be used to judge whether there is growth and biofilm of mucoid Pseudomonas aeruginosa formed the basis, and the expression of algU and algD have positive correlation.

【作者单位】：陕西省第二人民医院呼吸内科;西安交通大学医学院第二附属医院耳鼻咽喉头颈外科病院;
【基金】：陕西省科学技术厅基金(2012k17-03-04)
【分类号】：R440
【正文快照】： 本实验选取细菌培养证实的呼吸道铜绿假单胞菌感染病例,内镜下气管或支气管内取材,行生物膜形成相关基因及调节基因的检测。藻酸盐是铜绿假单胞菌生物膜胞外多聚基质的主要成分之一,algD基因对藻酸盐合成起关键作用,而mucA基因的突变可造成al-gD的去阻遏表达,使藻酸盐大量生成

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