Bt棉对AM真菌功能基因表达及代谢酶活性的影响

发布时间：2018-01-19 20:37

本文关键词： Bt棉 AM真菌基因表达酶活性　出处：《华中农业大学》2016年硕士论文　论文类型：学位论文

【摘要】：AM真菌广泛存在于土壤生态系统中,能与80%的陆生植物品种形成共生关系,对宿主植物矿质营养吸收(特别是磷)具有显著促进作用,同时AM真菌从宿主植物获得碳源。AM真菌与宿主植物的营养交换主要在共生结构体—丛枝界面进行,由一系列功能基因表达及酶的催化完成。磷转运蛋白基因Gint PT、碱性磷酸酶基因Gint ALP、单糖转运蛋白基因Gint MST2及碱性磷酸酶、酸性磷酸酶和脲酶在物质交换过程中发挥重要作用。AM真菌对宿主植物品种及环境条件的改变敏感。本研究通过盆栽实验,研究了我国广泛种植的5种转Bt基因棉对AM真菌R.irregularis共生特性、功能基因表达及关键酶活性的影响。得出的主要结论如下:⑴Bt棉对R.irregularis共生性有明显的影响。除中41外,其它4种Bt棉根段侵染率、侵染强度、丛枝丰度均低于对应的常规棉;Bt棉晋26、晋44和GK12丛枝面积低于其对应的常规棉;中41和s GK321丛枝面积与对应常规棉无显著差异。⑵Bt棉对侵染根段内AM真菌Gint PT、Gint ALP、Gint MST2基因表达量有明显影响。除个别时期外,Bt棉晋44、晋26、中41根段中Gint PT、Gint ALP、Gint MST2表达量低于对应常规棉;GK12根段中AM真菌基因表达量与其亲本泗棉3号差异不显著;而s GK321根段中Gint PT、Gint ALP、Gint MST2的表达量高于常规棉石远321。⑶Bt棉对共生体内碱性磷酸酶、酸性磷酸酶和脲酶活性有显著影响。除GK12外,其它Bt棉共生根段内碱性磷酸酶、酸性磷酸酶和脲酶活性表现为显著低于相应的常规棉或无显著性差异。⑷接种R.irregularis显著促进Bt棉和常规棉对磷的吸收,提高了宿主植物生物量,对氮吸收没有显著影响;Bt棉和常规棉间氮磷含量及生物量无显著的差异。
[Abstract]:AM fungi exist widely in the soil ecosystem and can form symbiotic relationship with 80% terrestrial plant varieties and play a significant role in promoting mineral nutrient uptake (especially phosphorus) of host plants. At the same time, the nutrient exchange between AM fungi and host plants was mainly carried out at the interface between symbiotic structure and branchlets. Phosphorous transporter gene Gint PTand alkaline phosphatase gene Gint ALP were expressed by a series of functional genes and catalyzed by enzymes. Monosaccharide transporter gene Gint MST2 and alkaline phosphatase. Acid phosphatase and urease play an important role in the process of substance exchange. AM fungi are sensitive to the change of host plant varieties and environmental conditions. The symbiotic characteristics of five transgenic BT cotton varieties against AM fungi R.irregularis were studied. The main conclusions are as follows: 1 BT cotton has a significant effect on R. irregularis symbiosis. The other four BT cotton had lower infection rate, infection intensity and branching abundance than the corresponding conventional cotton. The acreage of BT cotton Jin26, Jin44 and GK12 were lower than their corresponding conventional cotton. There was no significant difference between Zhong41 and s GK321 branch area and the corresponding conventional cotton. 2Bt cotton had no significant difference on AM fungus Gint ALP in infecting root segment. The expression of Gint MST2 gene was significantly affected except in a few periods, except in Jin44, Jin26, and Gint ALP in 41 segments of BT. The expression of Gint MST2 was lower than that of normal cotton. There was no significant difference between the gene expression of AM fungi and its parent Simian 3 in root segment of GK12. However, the expression of Gint MST2 in the root segment of s GK321 was higher than that in normal cotton Shiyuan 321.3Bt cotton. The activities of acid phosphatase and urease were significantly affected, except for GK12, alkaline phosphatase was found in the symbiotic root segment of BT cotton. The activity of acid phosphatase and urease was significantly lower than that of normal cotton or no significant difference .4 inoculated with R. irregularis significantly promoted the uptake of phosphorus by BT cotton and conventional cotton. The biomass of host plants was increased and nitrogen uptake was not significantly affected. There was no significant difference in nitrogen and phosphorus content and biomass between BT cotton and conventional cotton.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S562

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