番鸭细小病毒安徽分离株结构蛋白基因的克隆及序列分析

发布时间：2018-01-21 22:10

本文关键词： 番鸭细小病毒结构蛋白基因克隆序列分析　出处：《动物医学进展》2017年04期 　论文类型：期刊论文

【摘要】：为研究番鸭细小病毒(MDPV)安徽分离株的遗传变异特征,通过PCR扩增获得了MDPV结构蛋白(VP)基因全长序列AH-MDPV-VP,并将该序列与GenBank中登录的12条MDPV和鹅细小病毒(GPV)VP基因序列进行比对。结果显示,AH-MDPV-VP基因全长2 199bp,包括完整的VP1、VP2和VP3蛋白编码区。MDPV与GPV的VP基因部分序列一致,但具有明显的差异。进化分析显示,MDPV安徽分离株与基因重组型MDPV上海分离株SAAS-SHNH为同一分支,亲缘关系较近。同源性分析显示二者核苷酸序列同源性最高,为99.9%,且AH-MDPV-VP与GPV毒株SHFX1201的序列同源性也有89.5%。此外安徽分离株与其他MDPV的VP1、VP2和VP3基因同源性有逐步下降的趋势,而与GPV则相反呈上升趋势。进一步显示MDPV安徽分离株与基因重组型MDPV上海分离株SAAS-SHNH相似,可能为MDPV和GPV基因重组型水禽细小病毒。
[Abstract]:In order to study the genetic variation of muscovy duck parvovirus (MDPVV) isolated from Anhui Province, the full-length AH-MDPV-VP of MDPV structural protein was obtained by PCR amplification. The sequence was compared with 12 MDPV and goose parvovirus VP gene sequences registered in GenBank. The total length of AH-MDPV-VP gene was 2199bp.The complete VP2 and VP3 protein coding region of VP1VP2 and VP3 were identical to the VP gene of GPV. The evolutionary analysis showed that the Anhui strain was the same branch as the Shanghai strain of recombinant MDPV. The homology analysis showed that the nucleotide sequence homology was the highest (99.9%). The sequence homology of SHFX1201 between AH-MDPV-VP and GPV strain was 89.5. in addition, the VP1 of Anhui isolate was similar to that of other MDPV strains. The homology of VP2 and VP3 genes decreased gradually. The MDPV Anhui isolate was similar to the recombinant MDPV Shanghai isolate SAAS-SHNH. May be MDPV and GPV gene recombinant waterfowl parvovirus.
【作者单位】：安徽省农业科学院畜牧兽医研究所;
【基金】：安徽省农业科学院人才发展专项资金项目(17F0404) 安徽省家禽产业技术体系资金项目
【分类号】：S852.65
【正文快照】： 番鸭细小病毒(Muscovy duck parvovirus,MD-PV)属于细小病毒科细小病毒亚科依赖病毒属成员[1]。MDPV可引起番鸭的细小病毒病,该病是一种严重危害番鸭养殖业的急性传染性病,可通过多种方式传播,无明显的季节性,但冬春两季多发,1周龄~3周龄雏番鸭较为易感,一旦感染,发病率和病死

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