参与胡杨异形叶发生的发育相关基因表达模式及作用

发布时间：2018-01-21 23:20

本文关键词： 胡杨异形叶发育相关基因表达模式作用　出处：《基因组学与应用生物学》2017年11期 　论文类型：期刊论文

【摘要】：为了阐明胡杨异形叶发生的分子机制,本研究以胡杨披针形叶和宽卵形叶为材料,通过RNA-seq分析了上述两种叶形的基因表达谱,发现在胡杨异形中发生的4个时期中,共有3 991个基因在两种叶形中差异表达,利用权重基因共表达网络分析(WGCNA)发现一个含有583个基因的模块与叶形指数高度相关(r=0.93,p=0.004)。然后我们用Gene Ontology对这些基因的功能进行了注释和富集,发现其中有40个基因与发育密切相关。这40个基因的表达模式和功能分析表明,这些基因可以通过参与极性建成、细胞增殖、细胞生长、信号传导和代谢等生物学过程在胡杨异形叶发生中起重要作用。因此,我们的研究有助于进一步完善叶形发育的调控机制。
[Abstract]:In order to elucidate the molecular mechanism of heteromorphic leaves of Populus euphratica, the gene expression profiles of these two leaves were analyzed by RNA-seq using lanceolate leaves and broad ovate leaves of Populus euphratica as materials. A total of 3 991 genes were found to be differentially expressed in the two leaf shapes in the four stages in which the abnormal shapes of Populus euphratica occurred. A model containing 583 genes was found to be highly correlated with leaf shape index by using weighted gene coexpression network analysis (WGCNA). Then we used Gene Ontology to annotate and enrich the function of these genes. It was found that 40 genes were closely related to development, and the expression patterns and functional analysis of these 40 genes showed that these genes could be involved in polarity building, cell proliferation and cell growth. Biological processes such as signal transduction and metabolism play an important role in the development of heteromorphic leaves of Populus euphratica.
【作者单位】：塔里木大学生命科学学院;兵团塔里木盆地生物资源保护利用重点实验室;
【基金】：国家自然科学基金(No.31660298;No.31260275) 塔里木大学校长基金博士项目(TDZKBS201501)共同资助
【分类号】：Q943.2;S792.11
【正文快照】： *通讯作者,lifengz2011@126.comExpression Patterns and Functions of Genes Related to the Development ofPopulus euphratica Oliv.Heteromorphic Leaves MorphogenesisQin Shaowei1Li Cailin1Guo Zhongzhong1Bao Lianghong1Jiang Renjun1,2Zhang Na1Liu Zhanwen1Zhao Lif

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