大麦不同氮利用效率品种筛选及GS2基因的表达分析

发布时间：2018-01-22 00:35

本文关键词： 大麦 GS基因相对表达量氮高效利用　出处：《核农学报》2017年07期 　论文类型：期刊论文

【摘要】：为探明大麦品种的不同氮利用效率差异及质体型谷氨酰胺合成酶基因的表达差异,以48份大麦品种为材料,并利用不同氮水平营养液水培8周,研究其在低氮(0.4 mmol·L~(-1))、中氮(2.0 mmol·L~(-1))及高氮(5.0 mmol·L~(-1))下根、茎、叶的相关性状,筛选氮高效利用和氮低效利用的大麦品种,并利用qRT-PCR对筛选到的大麦氮高效和氮低效品种的GS2基因表达特性进行分析。结果表明,中氮下,除根干重和根含氮量外,其余的大麦苗期根、茎、叶各生物性状在品种间差异极显著,根据不同氮处理下的48份大麦品种苗期性状以及氮利用效率,筛选到1个氮高效品种(Z0099001)和2个氮低效品种(ARr-91和甘啤7号)。3个氮处理水平下,氮高效品种GS2基因的相对表达量高于氮低效品种,并且氮高效品种和氮低效品种根、茎、叶中GS2基因的相对表达量也各不相同,其中,叶中最高,茎中次之,根中最低。低氮条件下,根茎叶中,Z0099001的GS2基因的相对表达量高于甘啤7号,ARr-91最低。中氮条件下,根、茎、叶中均表现为氮高效品种高于氮低效品种,但在根中差异不显著,茎和叶中差异显著。高氮条件下,3个品种在根中GS2基因的相对表达量较低;在茎和叶中,Z0099001的相对表达量显著高于ARr-91和甘啤7号,且Z0099001叶中GS2基因的相对表达量在所有性状中最高。本研究结果为进一步解析大麦氮高效利用率奠定了基础。
[Abstract]:In order to find out the difference of nitrogen use efficiency and the expression of glutamine synthase gene in barley varieties, 48 barley varieties were cultured in water for 8 weeks with different nitrogen levels. The root and stem were studied at 0. 4 mmol 路L ~ (-1) of low nitrogen, 2.0 mmol 路L ~ (-1) of medium nitrogen and 5.0 mmol 路L ~ (-1) of high nitrogen. Leaf correlation traits were used to screen barley varieties with high nitrogen efficiency and low nitrogen efficiency. QRT-PCR was used to analyze the characteristics of GS2 gene expression in the selected barley varieties with high nitrogen efficiency and low nitrogen efficiency. The results showed that the roots of barley at seedling stage except root dry weight and root nitrogen content under medium nitrogen. The traits of stem and leaf were significantly different among varieties. According to the seedling characters and nitrogen utilization efficiency of 48 barley varieties treated with different nitrogen treatments. One nitrogen efficient cultivar (Z0099001) and two low nitrogen efficient varieties (Z0099001) and 2 low nitrogen varieties (ZARr-91 and Gan Beer No. 7) were selected under three nitrogen treatments. The relative expression amount of GS2 gene in nitrogen efficient variety was higher than that in low efficiency nitrogen variety, and the relative expression amount of GS2 gene in root, stem and leaf of nitrogen efficient variety and low nitrogen efficient variety were different, among which, the highest was in leaf. The relative expression of Z0099001 GS2 gene in rhizome leaves was higher than that in Gan Beer No. 7 ARr-91. Under medium nitrogen condition, root and stem were the lowest. The results showed that all the varieties with high nitrogen efficiency were higher than those with low nitrogen efficiency, but there was no significant difference in root, but there were significant differences between stems and leaves. Under the condition of high nitrogen, the relative expression of GS2 gene was lower in the root of the three varieties. The relative expression of Z0099001 in stem and leaf was significantly higher than that in ARr-91 and Gan Beer 7. The relative expression of GS2 gene in leaves of Z0099001 was the highest among all traits. The results laid a foundation for further analysis of nitrogen utilization efficiency in barley.
【作者单位】：甘肃农业大学/甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室;甘肃农业大学农学院;甘肃农业大学生命科学技术学院;
【基金】：国家自然基金(31171558,31460347) 农业产业技术体系建设项目(国家大麦青稞产业体系,CARS-05) 盛彤笙创新基金(GSAU-STS-1513)
【分类号】：Q943.2;S512.3
【正文快照】： 大麦(Hordeum vulgare L.)是世界上重要的粮食作物,也是生产啤酒的主要原料[1],其具有早熟、耐旱、耐瘠和耐盐等特点,在世界各地广泛种植[2]。氮是影响植物产量的最敏感元素,氮素吸收与积累是产量形成的重要基础,但是氮肥的过量使用会造成其利用率降低,增加氮素资源的无效浪费

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