微泡造影剂超声辐照介导自杀基因系统治疗肝癌实验研究

发布时间：2018-01-22 00:38

本文关键词： 基因治疗肝癌超声空化效应微泡造影剂　出处：《广东医学》2017年21期 　论文类型：期刊论文

【摘要】：目的探讨微泡造影剂超声辐照介导自杀基因系统治疗体内肝癌肿瘤的作用。方法采用小鼠肝癌瘤体内直接注射KDR-TK、AFP-TK基因和微泡造影剂SonoVue后加以超声辐照的介导方式,并通过给予GCV和5-FC两种前药,观察肿瘤生长体积、抑瘤率、生存时间、肿瘤细胞凋亡指标。结果 (1)治疗组小鼠皮下肝癌移植瘤体生长体积与对照组之间差异无统计学意义,但治疗组的抑瘤率有增加,与对照组比较差异有统计学意义(P0.05);(2)治疗组与对照组小鼠生存时间差异均无统计学意义(P0.05);(3)治疗组诱导肿瘤组织内的细胞凋亡增多,与对照组差异有统计学意义(P0.05);(4)各组除针道外,肿瘤组织内未见明显坏死。结论微泡造影剂超声辐照介导自杀基因治疗系统具有抑制小鼠体内肝癌实体瘤的生长速率的趋势,可能的机制是诱导肿瘤细胞凋亡。
[Abstract]:Objective to investigate the effect of microbubble contrast agent ultrasound irradiation mediated suicide gene system in the treatment of hepatocellular carcinoma in vivo. Methods KDR-TK was injected directly into mice hepatoma. AFP-TK gene and microbubble contrast agent SonoVue were induced by ultrasound irradiation. The tumor growth volume, tumor inhibition rate and survival time were observed by GCV and 5-FC prodrugs. Results: there was no significant difference in tumor growth volume between the subcutaneous liver cancer transplantation group and the control group, but the tumor inhibition rate in the treatment group was higher than that in the control group. Compared with the control group, the difference was statistically significant (P 0.05). (2) there was no significant difference in survival time between the treatment group and the control group (P 0.05). 3) the apoptosis in tumor tissue was increased in the treatment group, which was significantly different from that in the control group (P 0.05). Conclusion the suicide gene therapy system mediated by ultrasound with microbubble contrast agent has a tendency to inhibit the growth rate of solid tumor of liver cancer in mice. The possible mechanism is to induce apoptosis of tumor cells.
【作者单位】：广州医科大学附属第一医院超声科;中山大学附属第一医院检验科;
【基金】：广东省科技计划项目(编号:2014A020212606)
【分类号】：R735.7
【正文快照】： 单纯疱疹病毒Ⅰ型的胸腺激酶基因(HSV-TK)/更昔洛韦(GCV)是目前发展成熟的自杀基因/前体药物治疗体系。基因治疗首先必须将目的基因转染靶细胞,目前在临床实际应用中最大的障碍依然是缺乏安全有效的基因转染方式,例如病毒载体,体外的实验虽具有转染效率高的优点,但用于人体可

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