角毛壳菌丝切蛋白基因的克隆和特性分析

发布时间：2018-01-22 03:00

  本文关键词： 角毛壳菌 丝切蛋白 基因克隆 生物信息学分析　出处：《河南师范大学学报(自然科学版)》2017年03期 　论文类型：期刊论文

【摘要】：在构建的角毛壳菌(Chaetomium cupreum)菌丝体cDNA文库中获得丝切蛋白(Cofilin)的ESTs序列片段,为研究角毛壳菌的生物特性,提高其生物防治效果,以角毛壳菌总RNA反转录产物为模板进行PCR扩增,成功获得了Cofilin基因cDNA序列.生物信息学分析结果表明,该基因全长468bp,编码155个氨基酸,编码蛋白理论分子量17kD,等电点是4.99,α-螺旋和无规则卷曲是其主要的二级结构,为亲水性蛋白质.该蛋白有6个位点发生Ser磷酸化,4个位点发生Tyr磷酸化,有4个保守区,ClustalX分析说明该蛋白与柄孢霉(Podospora anserina)和球毛壳菌(C.g lobosum)亲缘关系较近.
[Abstract]:The ESTs sequence fragment of Cofilin was obtained from the constructed cDNA library of Chaetomium cupreum. In order to study the biological characteristics of Trichoderma keratoderma and improve its biological control effect, the total RNA reverse transcription product was used as template for PCR amplification. The cDNA sequence of Cofilin gene was successfully obtained. Bioinformatics analysis showed that the full length of the gene was 468 BP, encoding 155 amino acids, and the theoretical molecular weight of encoding protein was 17 KD. The isoelectric point is 4.99, and 伪 -helix and irregular crimp are the main secondary structures, which are hydrophilic proteins. There are 6 sites of Ser phosphorylation and 4 sites of Tyr phosphorylation. There are four conservative areas. ClustalX analysis showed that the protein was closely related to Podospora anserina and C. G lobosum.
【作者单位】： 河南大学生命科学学院;
【基金】：国家自然科学基金(31400108) 河南大学科研项目(xxjc20140009)
【分类号】：S476
【正文快照】： 角毛壳菌(C.cupreum)属于子囊菌门盘菌亚门毛壳菌属真菌,是一种生物防治真菌,主要通过产生毒素类物质或者生长优势等方式抑制植物病原真菌的生长,对一些常见的植物病原真菌有较好的防治效果,如:苹果炭疽菌(Colletotrichum gloeosporioides)、番茄叶霉菌(Cladosporium fulvum),，

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