水稻粒型基因GW2和GS3遗传效应分析

发布时间：2018-01-22 03:32

本文关键词： 水稻 GW基因 GS基因粒型籽粒质量　出处：《江苏农业学报》2017年04期 　论文类型：期刊论文

【摘要】：以大粒品种TD70和小粒品种Kasalath为背景材料,分别对其中的GW2和GS3基因进行了反义和超表达载体的构建和转化。结果表明,Kasalath来源的GW2(GW2~K)和GS3(GS3~K)转化TD70的超表达材料籽粒质量均降低,且GW2~K的贡献大于GS3~K;GW2~K和GS3~K转化Kasalath的反义表达材料籽粒质量均增加,GW2~K的贡献大于GS3~K;TD70来源的GW2(GW2~T)转化Kasalath的超表达材料千粒质量显著增加,而TD70来源的GS3(GS3~T)转化Kasalath的超表达材料粒型和籽粒质量没有显著变化,说明不同遗传背景中GW2和GS3的基因效应有差异,但同一背景下GW2对籽粒质量的贡献大于GS3。
[Abstract]:The antisense and super-expression vectors of GW2 and GS3 genes were constructed and transformed from large grain TD70 and small grain Kasalath respectively. GW2GW2K) and GS3GW3K) transformed into TD70 decreased in grain quality, and the contribution of GW2~K was greater than that of GS3K. The grain quality of antisense expression materials transformed by GW2~K and GS3~K increased the contribution of GW2K to GS3K. GW _ 2 GW _ (2) T) derived from TD70 significantly increased the weight of thousands of super-expression materials for transforming Kasalath into GW _ (2) G _ (2) G _ (2) T. However, there was no significant change in grain type and grain quality in the transformation of Kasalath from GS3 / GS3Tfrom TD70. The results showed that the gene effects of GW2 and GS3 were different in different genetic backgrounds, but the contribution of GW2 to grain quality was greater than that of GS3 under the same background.
【作者单位】：南京农业大学农学院/江苏省现代作物生产协同创新中心;江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心;
【基金】：公益性行业科研专项(201603002-5-1) 现代农业产业技术体系建设专项基金项目(CARS-01-47) 江苏省重点研发计划项目(BE2016370)
【分类号】：S511
【正文快照】： 水稻是主要粮食作物之一,对粒型或籽粒质量基因遗传效应的分析有助于提高水稻产量。近年来,国内外学者纷纷克隆出控制水稻粒型的基因,如GW2[1]、GS3[2-3]、q GL3[4]、GS5[5]、GW5[6-8]、TGW6[9]、GW8[10]等。GW2是一个控制粒宽和粒质量的主效基因,GW2编码的环型E3泛素连接酶位

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