原发性肝癌B细胞异位基因2的表达及临床意义

发布时间：2018-01-22 05:43

本文关键词： 肝细胞癌 BTG 总生存期免疫组化预后　出处：《第三军医大学学报》2017年07期 　论文类型：期刊论文

【摘要】：目的探讨B细胞异位基因2(B cell translocation gene 2,BTG2)在原发性肝细胞癌中的表达及与术后生存期之间的关系。方法共纳入125例可手术切除的原发性肝细胞癌患者,其中有完整随访记录101例;用免疫组化方法检测患者肿瘤组织中BTG2蛋白的表达,并分为3组:BTG2高表达组,BTG2蛋白中表达(++)或强表达(+++);BTG2低表达组,BTG2蛋白低表达(+);BTG2阴性组,BTG2蛋白无表达(-)。分析BTG2表达水平与临床病理因素和术后总生存期的相关性。选取12对癌与癌旁配对组织,用RT-PCR检测其BTG2表达水平。结果 125例癌组织中,BTG2阴性组、低表达组和高表达组分别为106、13、6例;癌旁组织中,BTG2阴性组、低表达组和高表达组分别为41、14、22例,癌与癌旁组织BTG2表达差异有统计学意义(χ~2=28.102,P0.001)。RT-PCR检测结果显示12对癌与癌旁配对组织中,癌组织BTG2表达水平低于癌旁的有8例。癌栓发生与BTG2表达有明显相关性(χ~2=8.305,P=0.013),其中低表达组癌栓发生率最高。BTG2低表达组中位总生存期为36个月(95%CI:8.437~63.564),而高表达和阴性表达组未达到中位总生存期(Log-Rankχ~2=4.512,P=0.105)。亚组分析表明,BTG2高表达组患者总生存期显著长于低表达组(χ~2=4.266,P=0.039),而高表达与阴性组(χ~2=2.729,P=0.099)以及低表达与阴性组(χ~2=1.400,P=0.237)之间差异没有统计学意义。逐步Cox回归分析结果显示,分化程度、肝硬化、癌栓和转移情况是总生存期的独立预后因素。结论 BTG2蛋白在原发性肝细胞癌中的表达呈低表达,并且BTG2蛋白的高表达提示肝癌患者预后较好,可作为肝细胞癌的潜在预后指标。
[Abstract]:Objective to investigate the effect of B cells (B cell translocation 2 ectopic gene gene 2, BTG2) expression in primary hepatocellular carcinoma and the relationship between the survival period and after operation. Methods a total of 125 patients with resectable hepatocellular carcinoma patients, which have complete follow-up records of 101 cases; the expression of BTG2 protein using immunohistochemical method in tumor tissue, and divided into 3 groups: BTG2 high expression group, the expression of BTG2 protein in (+ +) or (+ + +); the strong expression of BTG2 low expression group and low expression of BTG2 (+); BTG2 negative group, BTG2 protein expression (-) correlation analysis. The expression level of BTG2 and clinical pathological factors and postoperative overall survival. Select 12 of cancer and adjacent tissue, the expression level of BTG2 was detected by RT-PCR. Results 125 cases of cancer tissues, BTG2 negative group, low expression group and high expression group were 106,13,6; tumor tissues, BTG2 negative group low expression and high expression group. Group were 41,14,22, carcinoma and adjacent tissue BTG2 expression was statistically significant (~2=28.102, P0.001).RT-PCR showed that 12 of cancer and adjacent tissues, the level of the 8 cases was lower than that in adjacent cancer tissues. The expression of BTG2 tumor thrombus occurrence and the expression of BTG2 is significantly correlated (x ~2=8.305, P=0.013), the highest incidence of cancer embolus group.BTG2 low expression group median overall survival was 36 months (95%CI:8.437~63.564), the low expression and high expression and negative expression group did not reach the median overall survival (Log-Rank x ~2=4.512, P=0.105). Subgroup analysis showed that high expression of BTG2 in patients with overall survival significantly longer than the low expression group (~2=4.266, P=0.039), and the high expression and negative group (~2=2.729, P=0.099) and low expression and negative group (~2=1.400, P=0.237) between the difference was not statistically significant. Gradually the results were analyzed with Cox regression showed that the degree of differentiation, cirrhosis, and cancer embolus Metastasis is an independent prognostic factor of overall survival. Conclusion the expression of BTG2 protein is low in primary hepatocellular carcinoma, and the high expression of BTG2 indicates that the prognosis of HCC is good. It can be used as a potential prognostic indicator for hepatocellular carcinoma.

【作者单位】：第三军医大学大坪医院野战外科研究所肿瘤中心;
【基金】：国家自然科学基金面上项目(81572959)~~
【分类号】：R735.7
【正文快照】： 原发性肝癌是我国最常见的肿瘤之一,居所有肿瘤发病率的第4位,死亡率居第3位[1]。肝癌按细胞分型可分为肝细胞癌、胆管细胞癌及混合型肝癌,其中肝细胞癌最常见。肝细胞癌(hepatocellular carcinoma,HCC)是预后较差的肿瘤之一,约90%的患者有慢性乙型肝炎病史。由于只有10%~20%

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