肿瘤干细胞中受H3K9me2调控的干性基因的筛选

发布时间：2018-01-22 08:46

  本文关键词： ChIP-seq Ga HKme Bix U细胞株　出处：《生物技术通报》2016年12期 　论文类型：期刊论文

【摘要】：为了分析比较甲基转移酶G9a和组蛋白H3K9me2修饰在胶质瘤干细胞与非干细胞中存在的差异,筛选出维持胶质瘤干细胞干性的相关基因。通过G9a抑制剂促进U87细胞成球和过表达G9a促进U87细胞分化的方法,培养了成球的胶质瘤干细胞和贴壁的非干细胞,这两种细胞的CD133表达差异明显。再利用H3K9me2抗体通过Ch IP-seq技术比较H3K9me2修饰在干细胞组与非干细胞组中的差异,在存在差异的基因中,对TSS±2 000 bp范围内的基因进行了GO分析,并随机选出10个转录因子进行QPCR验证,结果与Ch IP-seq实验基本一致。
[Abstract]:To compare the difference of methyltransferase G9a and histone H3K9me2 in glioma stem cells and non-stem cells. The genes related to maintaining stem cell dryness of glioma were screened. G9a inhibitor was used to promote the formation of U87 cells and overexpression of G9a to promote the differentiation of U87 cells. Glioma stem cells and adherent non-stem cells were cultured. H3K9me2 antibody was used to compare the difference of H3K9me2 modified H3K9me2 between stem cell group and non-stem cell group by Ch IP-seq technique. Among the differentially expressed genes, go was used to analyze the genes in the range of TSS 卤2 000 BP, and 10 transcription factors were randomly selected for QPCR validation. The results were consistent with Ch IP-seq experiment.
【作者单位】： 宁夏医科大学基础医学院生理学系;宁夏颅脑疾病重点实验室国家重点实验室培育基地;
【基金】：国家自然科学基金(31260246,31460300) 宁夏医科大学校级项目(XY201406)
【分类号】：R730.2
【正文快照】： 自我复制是干细胞所特有的特性,在保持高度增殖能力的同时还能维持子代细胞处于不分化状态,并保持其多向分化潜能。肿瘤干细胞广泛存在于各种肿瘤中[1-6],也具有自我复制和多向分化的特性,并表达多种干细胞的标志分子,如CD34、CD133、ALDH1、CXCR4、Nestin、LRCs等。肿瘤干细，

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