大叶落地生根KdFBX基因的cDNA克隆与表达分析

发布时间：2018-01-22 08:58

本文关键词： 大叶落地生根 F-box蛋白基因表达　出处：《农业生物技术学报》2017年12期 　论文类型：期刊论文

【摘要】：F-box蛋白作为SCF(Skp1-Cullin1-F-box)复合体的成员,参与植物生长发育、细胞分裂及植物激素的响应等调控过程。为深入了解大叶落地生根(Kalanchoe daigremontiana)中胎生苗发育的分子机制,利用RACE-PCR技术从大叶落地生根中克隆了1个新的基因Kd FBX(Gen Bank No.KU740356)。该基因具有328个氨基酸残基,分子量为37.72 k D,等电点为4.4,开放阅读框(open reading frame,ORF)长度为987 bp。其蛋白与亚洲棉(Asiatic cotton)、蓖麻(Ricinus communis)等植物的同源性较高,与拟南芥(Arabidopsis thaliana)的进化关系相对接近。实时荧光定量PCR分析表明,该蛋白基因在大叶落地生根的根中表达量最高,且在渗透胁迫(甘露醇)处理过程中下调表达。本研究首次从大叶落地生根中克隆出Kd FBX基因,为进一步探究该基因的功能提供理论依据。
[Abstract]:F-box protein, as a member of SCFN-Skp1-Cullin1-F-box) complex, is involved in plant growth and development. In order to understand the molecular mechanism of embryogenic seedling development in Kalanchoe daigremontiana, cell division and phytohormone response were studied. A new gene KD FBX(Gen Bank no. KU740356 was cloned from rooting of large leaf by RACE-PCR. The gene has 328 amino acid residues. The molecular weight is 37.72 KD, the isoelectric point is 4.4, and the open reading frame is open reading frame. The length of ORF was 987 bp.The protein had high homology with Asiatic cottonus and Ricinus communis. The evolutionary relationship between Arabidopsis thaliana and Arabidopsis was close to that of Arabidopsis thaliana. The expression of the protein gene was the highest in the rooting roots and down-regulated during osmotic stress (mannitol). In this study, KD FBX gene was first cloned from the rooting of Lymphoma macrophylla. To provide a theoretical basis for further exploring the function of the gene.
【作者单位】：深圳市日f园林绿化有限公司;北京林业大学林学院草坪研究所;华南农业大学林学与风景园林学院草业科学系/广东省草业工程技术研究中心;
【基金】：深圳市科技计划项目(No.CXZZ20140418110342522) 北京林业大学与厦门日懋城建园林建设股份有限公司产学研合作项目
【分类号】：Q943.2;S682.33
【正文快照】： 草业科学系/广东省草业工程技术研究中心,广州510642c DNA Cloning and Expression Analysis of Kd FBX Gene in KalanchoedaigremontianaDUAN Xue-Jiao1LIANG Xiao-Hong2GE Yong-Qiang1OU Su-Rong1ZHONG Tian-Xiu3*1 Shenzhen Risheng Landscape Company Limited,Shenzhen 51

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