一个与烟草TMV抗性基因N紧密连锁的共显性SSR标记

发布时间：2018-01-22 23:19

  本文关键词： 烟草 烟草花叶病 分离集团分组分析法 共显性SSR标记　出处：《中国烟草学报》2017年02期 　论文类型：期刊论文

【摘要】：烟草普通花叶病(Tobacco Mosaic Virus,TMV)是烟叶生产的主要病害,为建立烟草抗TMV分子标记辅助选择体系,本研究以高抗TMV烤烟品种Coker176和易感TMV烤烟品种Y3以及它们的BC1F1群体为试材,采用分离集团分组分析法(Bulked Segregation Analysis,BSA)和简单序列重复(Simple Sequence Repeat,SSR)标记技术,筛选与TMV抗性基因N紧密连锁的共显性SSR标记。通过对18764对SSR引物的分析表明,有396对SSR引物在两亲本间有多态,选出1对SSR引物TM508-007扩增出的标记与TMV抗性的N基因紧密连锁,遗传连锁距离为0.41 c M,证实获得的与TMV抗性基因N紧密连锁的共显性SSR标记稳定可靠,可用于烟草抗TMV分子标记辅助选择。
[Abstract]:Tobacco Mosaic virus (TMV) is a major disease in tobacco leaf production. It is a molecular marker-assisted selection system for tobacco resistance to TMV. In this study, high resistant TMV flue-cured tobacco varieties Coker176 and susceptible TMV varieties Y3 and their BC1F1 populations were used as the test materials. The separated Segregation Analysis was analyzed by grouping analysis of separated groups. BSA) and simple Sequence repeat SSRs. Screening codominant SSR markers closely linked to N of TMV resistance gene. The analysis of 18764 pairs of SSR primers showed that there were 396 pairs of SSR primers with polymorphism between the two parents. A pair of SSR primers TM508-007 was selected to amplify the marker closely linked to the N gene of TMV resistance, and the genetic linkage distance was 0.41 cm. It was proved that the codominant SSR markers closely linked to the TMV resistance gene N were stable and reliable and could be used in the assisted selection of tobacco TMV resistant molecular markers.
【作者单位】： 湖南农业大学生物科学技术学院;云南省烟草农业科学研究院 烟草生物技术育种重点实验室国家烟草基因工程研究中心;山东中烟工业有限责任公司;
【基金】：中国烟草总公司云南省公司科技项目(2016YN27)
【分类号】：S435.72
【正文快照】： 烟草普通花叶病是由烟草花叶病毒(Tobacco的全部基因型,即上述显性标记只能分辨出材料中是Mosaic Virus,TMV)引起的,TMV是第一个被鉴定否含有抗性基因N,而无法进一步地区分出纯合抗性的单链正义病毒[1],属于花叶病毒家族[2],具有传(NN)和杂合抗性(Nn)。有部分标记是基于N基染，

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