大恒鸡与金阳丝毛鸡TGFB2基因在不同组织中的表达差异

发布时间：2018-01-24 12:28

本文关键词： 鸡 TGFB基因定量PCR 组织表达　出处：《四川农业大学学报》2017年01期 　论文类型：期刊论文

【摘要】：【目的】探讨TGFB2基因对鸡肌肉生长发育的影响。【方法】利用定量PCR方法对TGFB2基因在鸡不同组织、不同生长发育阶段的表达差异进行了比较研究。【结果】研究结果显示:2周龄大恒鸡腿肌中TGFB2 mRNA表达量显著高于金阳丝毛鸡,并且达到最高值。10周龄金阳丝毛鸡母鸡胸肌组织中TGFB2 mRNA表达量均显著高于大恒鸡,并达到最高值;大恒鸡公鸡10周龄腿肌组织中TGFB2 mRNA表达量均与其他周龄差异显著,并达到最高值。金阳丝毛鸡、大恒鸡母鸡10周龄胸肌组织中TGFB2 mRNA表达量均显著高于2周龄。【结论】TGFB2基因可能是影响鸡肌肉生长发育的候选基因。
[Abstract]:[objective] to investigate the effect of TGFB2 gene on the growth and development of chicken muscle. [methods] quantitative PCR method was used to detect the expression of TGFB2 gene in different tissues of chicken. [results] the results showed that the expression of TGFB2 mRNA in the legged muscle of two weeks old Dacheng chicken was significantly higher than that in the golden silk feather chicken at the age of 2 weeks. The expression of TGFB2 mRNA in breast muscle of Jinyang hairy hen at 10 weeks of age was significantly higher than that of Daheng chicken, and reached the highest value. The expression of TGFB2 mRNA in 10 week old leg muscle of Dacheng chicken was significantly different from that of other weeks old, and reached the highest value. The expression of TGFB2 mRNA in the breast muscle of 10 weeks old hens was significantly higher than that of 2 weeks old. [conclusion] TGFB2 gene may be a candidate gene to affect the growth and development of chicken muscle.
【作者单位】：四川省畜牧科学研究院;四川大恒家禽育种有限公司;动物遗传育种四川省重点实验室;
【基金】：四川省科技支撑计划(2015NZ0101) 四川省基本科研业务专项(SASA2014A10) 四川省科研院所成果转化项目(15010126)
【分类号】：S831
【正文快照】： 3.动物遗传育种四川省重点实验室,成都610066)转化生长因子-β(transforming growth factor-β,TGF-β)是一组蛋白超家族。TGF-β的命名是根据这种细胞因子能使正常的成纤维细胞的表型发生转化,即在表皮生长因子(EGF)同时存在的条件下,改变成纤维细胞巾壁生长特性而获得在琼脂

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