鸡柔嫩艾美耳球虫海南株SO7基因表达产物的免疫保护效果观察

发布时间：2018-01-24 11:56

本文关键词： SO基因柔嫩艾美耳球虫海南株表达产物免疫效果　出处：《中国家禽》2017年03期 　论文类型：期刊论文

【摘要】：为研究鸡柔嫩艾美耳球虫(Eimeria tenella,E.tenella)海南株SO7基因原核表达产物对E.tenella攻击的免疫保护效力,分别于8和16日龄采用SO7基因可溶性重组蛋白和包涵体重组蛋白经肌肉注射两次免疫文昌鸡公雏,同时设立攻虫对照组和空白对照组;其中,免疫组和攻虫对照组雏鸡于27日龄接种5×10~4个E.tenella卵囊,观察其诱导产生的保护力。结果显示,与攻虫对照组相比,SO7可溶性蛋白组雏鸡的相对卵囊产量降低了82.9%,攻虫至试验结束的增重增加了76.3%,盲肠病变减少了29.8%;SO7包涵体蛋白组雏鸡的相对卵囊产量降低了60.4%,攻虫至试验结束鸡增重增加了42%,盲肠病变减少了18.7%。结果表明,E.tenella海南株SO7基因在大肠杆菌中的表达产物对鸡柔嫩艾美耳球虫的攻击有一定的免疫保护作用,且SO7可溶性蛋白的抗球虫效力优于包涵体蛋白。
[Abstract]:To study Eimeria tenella of Eimeria tenella. The prokaryotic expression products of SO7 gene of E. tenella-Hainan strain could protect against E. tenella attack. The soluble recombinant protein of SO7 gene and the recombinant protein of inclusion body were injected intramuscularly into the male chicks of Wenchang twice at 8 and 16 days of age respectively. The control group and the blank control group were set up at the same time. The immune group and the control group were inoculated with 5 脳 10 ~ 4 E. tenella oocysts at the age of 27 days to observe the protective effect of E. tenella. The results showed that compared with the control group, the immune group and the control group were inoculated with 5 脳 10 ~ 4 E. tenella oocysts. In SO7 soluble protein group, the relative egg sac yield decreased by 82.9%, the weight gain increased by 76.3% and the cecum lesion decreased by 29.8%. The relative egg sac yield of chicks in SO7 inclusion body protein group was reduced by 60.4. The weight gain increased by 42 and the cecum lesion decreased by 18.7by the end of the experiment. The expression product of SO7 gene of E. tenella Hainan strain in Escherichia coli had a protective effect on the attack of E. tenella Hainan strain Eimeria tenella. SO7 soluble protein was superior to inclusion body protein in anti-coccidiosis activity.
【作者单位】：海南省农业科学院畜牧兽医研究所;海南省热带动物繁育与疫病研究重点实验室;
【基金】：海南省自然科学基金项目(311042) 现代农业产业技术体系建设专项资金(CARS-42-z19) 海南省农业科学院农业科技创新专项(琼农财[2013]32号)
【分类号】：S858.31
【正文快照】： 鸡球虫病是由一种或多种艾美耳属球虫寄生于肠道特定部位的上皮细胞而引起的一类细胞内寄生虫病,在养鸡生产中较为常见,危害较大。据统计,全世界因球虫病造成的经济损失达数30亿美元[1~3]。传统的鸡球虫病控制主要依赖于药物预防。由于药物防治中耐药性和药物残留两大问题的日

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