水牛IGF1基因多态性与其生长性状、血清浓度以及IGF1和IGF1R基因表达的相关性研究（英文）

发布时间：2018-01-24 08:10

本文关键词： 胰岛素样生长因子(IGF) 胰岛素样生长因子受体(IGFR) 单核苷酸多态性(SNP) 生长性状水牛　出处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年12期 　论文类型：期刊论文

【摘要】：目的:探讨胰岛素样生长因子1(insulin-like growth factor1,IGF1)基因单核苷酸多态性(SNP)与埃及水牛生长性状的关联性。创新点:发现IGF1基因多态性对埃及水牛的生长性状、血液生化指标和基因表达有显著影响,并为埃及水牛的选育提供重要的分子遗传标记。方法:以200头埃及水牛为试验对象,对其IGF1基因的多态性进行检测,并分析其与生长性状、血液生理生化指标和基因表达的相关性。结论:在水牛IGF1基因多态性分析中,发现两个新的SNP位点(G64A和G280A)分别分布在外显子1和外显子4的非编码区。不同基因型的统计分析表明,G64A和G280A位点的GG基因型水牛个体分别在3~6月龄和6~9月龄的体重(BW)和平均日增重(ADG)均显著高于其他基因型(P0.05)。这两个SNPs位点的组合基因型产生了三种单倍体GG/GG、AG/AG和AA/AA。在3~12月龄的水牛个体中,单倍体基因型与BW和ADG存在显著关联(P0.0001)。纯合的GG/GG单倍体基因型水牛生长性能优于其他水牛。两个SNP位点与半腱肌中IGF1和IGF1R的mRNA水平以及IGF1血清浓度水平相关。此外,GG/GG单倍体水牛表现出较高的mRNA和血清浓度水平。综上所述,这两种SNP位点G64A和G280A可作为埃及水牛生长性状选育的重要分子遗传标记。
[Abstract]:Objective: To investigate the effects of insulin-like growth factor 1 (insulin-like growth, factor1, IGF1) gene single nucleotide polymorphisms (SNP) associated with growth traits. The Egyptian buffalo Innovation: IGF1 gene polymorphisms on growth traits of Egypt Buffalo, blood biochemical index and gene expression have a significant effect, provide an important molecular genetic markers and breeding of Egyptian buffalo. Methods: 200 head of buffalo Egypt as test object, detect the polymorphism of the IGF1 gene, and to analyze its correlation with growth traits, physiological and biochemical indexes of blood and gene expression. Conclusion: in the analysis of buffalo IGF1 gene polymorphism in the discovery of two new SNP loci (G64A and G280A) respectively in non encoding region of exon 1 and exon 4. Statistical analysis showed that different genotypes, G64A and G280A loci GG genotype individual buffalo respectively in 3~6 months and 6~9 months weight (BW) And the average daily gain (ADG) were significantly higher than those of other genotypes (P0.05). The combined genotypes of two SNPs loci produced three haploid GG/GG, individual buffalo at the age of 3~12 months, AG/AG and AA/AA., BW and ADG haploid genotype and significant association (P0.0001). The GG/GG genotype of haploid buffalo pure growth performance is better than other buffalo. IGF1 and IGF1R two SNP loci and semitendinosus muscle in mRNA level and IGF1 level of serum. In addition, the buffalo GG/GG haplotype exhibited higher mRNA and serum level. To sum up, the two SNP loci G64A and G280A can be used as an important molecular growth traits of Egypt buffalo the selection of genetic markers.

【作者单位】： Department
【基金】：Project supported by the Science Technology Development Fund(STDF,No.2585),Ministry of Scientific Research,Egypt
【分类号】：S823.83
【正文快照】： 1 IntroductionInsulin-like growth factor 1(IGF1)is essentialfor activation of mitogenic proliferation and myoblastdifferentiation in skeletal muscles(Werner et al.,1994;Oksbjerg et al.,2004)and therefore it can beused in marker-assisted selection(MAS)for

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