水稻垩白基因Chalk5功能标记的开发与应用

发布时间：2018-01-25 01:09

本文关键词： 水稻垩白 Chalk 等位基因特异PCR 功能标记Q　出处：《华北农学报》2017年01期 　论文类型：期刊论文

【摘要】：水稻垩白是衡量水稻外观品质优劣的重要指标之一,降低稻米的垩白是培育优质水稻品种的前提。Chalk5是一个能显著降低稻米垩白从而提高稻米外观品质的主效基因。为提高垩白基因Chalk5在育种中的选择效率,在前人克隆的基础上,根据其启动子区域存在的2个功能突变位点,分别设计等位基因特异PCR(ARMS-PCR)引物,最终筛选出2对Chalk5基因的功能标记Chalk12、NChalk12和Chalk22、NChalk22,通过PCR产物测序的方法对这2对标记的检查结果进行了验证,说明Chalk12、NChalk12和Chalk22、NChalk22可以准确鉴定出Chalk5的不同基因型。利用该标记对不同来源的43份籼稻资源、江苏2007-2013年审定的65份粳稻品种和太湖流域179份粳稻地方资源进行了Chalk5基因型检测,筛选到携带chalk5低垩白率等位基因的籼稻资源20份,太湖流域粳稻地方资源仅8份,其余259份粳型资源中均不携带chalk5低垩白率等位基因,这也说明chalk5主要分布在籼型水稻资源中,在粳型资源中分布很少。
[Abstract]:Rice chalkiness is one of the important indexes to evaluate the appearance quality of rice. Chalk5 is a major gene that can significantly reduce chalkiness and improve the appearance quality of rice. In order to improve the chalkability gene Chalk5 in breeding, chalk5 is a major gene that can reduce chalkiness significantly and improve the appearance quality of rice. The selection efficiency in. On the basis of previous cloning, allele-specific primers were designed according to the two functional mutation sites in the promoter region. Finally, two functional markers of Chalk5 gene, Chalk12, NChalk12 and Chalk22, were screened out. The results of the two pairs of markers were verified by PCR sequencing, indicating that Chalk12, NChalk12 and Chalk22. Different genotypes of Chalk5 could be identified by NChalk22, and 43 varieties of indica rice from different sources could be identified by this marker. Chalk5 genotypes of 65 japonica rice varieties and 179 japonica rice local resources from 2007 to 2013 in Jiangsu Province were detected. Twenty indica rice resources with low chalkiness rate allele of chalk5 and only 8 japonica rice native resources in Taihu Basin were screened. None of the remaining 259 japonica resources carried low chalkiness rate alleles of chalk5, which indicated that chalk5 was mainly distributed in indica rice resources and rarely in japonica rice resources.
【作者单位】：江苏省农业科学院粮食作物研究所国家水稻改良中心南京分中心;扬州大学江苏省粮食作物现代产业技术协同创新中心;苏州农业职业技术学院;扬州大学生物科学与技术学院;
【基金】：国家自然科学基金项目(31400175) 江苏省自然科学基金项目(BK20130725) 江苏省现代农业研发项目(BE2015355) 江苏科技成果转化资金(BA2014134) 公益性行业科研专项(201303102) 扬州市农业前瞻性研究项目(YZ2014165)
【分类号】：S511
【正文快照】： 水稻是我国重要的粮食作物,种植面积和总产都位居第一。随着人们生活水平的提高,消费者对稻米品质需求越来越高。稻米品质主要包括加工品质、外观品质、蒸煮食味品质和营养品质,具体内容有:透明度、垩白、粒型、直链淀粉含量、胶稠度、糊化温度、蛋白质含量以及有无香味等。其

【相似文献】