在患CyHV-2病的异育银鲫肠道黏膜中胆固醇、胆汁酸代谢通路基因的差异表达

发布时间：2018-01-25 01:26

本文关键词： 氧化鱼油黏膜胆固醇胆汁酸异育银鲫　出处：《水生生物学报》2017年05期 　论文类型：期刊论文

【摘要】：为了探讨CyHV-2疾病条件下异育银鲫胆汁酸肠肝循环代谢途径主要基因表达活性的变化,以患CyHV-2病的、正常的异育银鲫肠道黏膜为材料,提取总RNA,采用RNA-Seq测序、对单一基因进行注释,并进行KEGG富集分析、单一基因差异表达分析。结果显示,肠道黏膜组织有7770个基因显著性差异表达,其中,差异表达上调基因数为3335个、差异表达下调的基因数为4435个,表明CyHV-2病的发生对肠道黏膜组织基因表达产生了重大的影响。病鱼胆固醇、胆汁酸生物合成代谢途径的酶、蛋白质的基因显著性差异表达,显示肠道黏膜胆固醇、胆汁酸合成代谢受到显著性影响。参与胆固醇、胆汁酸合成代谢调节作用,以及胆固醇、胆汁酸分泌、吸收、转运等生理过程的蛋白质、酶的基因也是差异表达下调,胆汁酸肠肝循环有出现代谢障碍的趋势。结果表明,CyHV-2病的发生对肠道黏膜组织基因表达产生了严重影响,对胆固醇、胆汁酸的合成代谢途径、胆汁酸肠肝循环途径的基因表达产生了严重影响,将导致病鱼体内胆固醇、胆汁酸量的不足。患CyHV-2病病鱼血清胆汁酸含量下降了99%、胆固醇含量下降了10%,证实了上述基因差异表达的趋势。
[Abstract]:In order to investigate the changes of the main gene expression activity of intestinal circulatory metabolism pathway of bile acid in silver crucian carp under the condition of CyHV-2 disease, the intestinal mucosa of normal allogeneic crucian carp with CyHV-2 disease was used as the material. Total RNAs were extracted, RNA-Seq sequencing was used to annotate the single gene, KEGG enrichment analysis was carried out, and the differential expression analysis of single gene was carried out. There were 7770 genes differentially expressed in intestinal mucosa, of which 3335 genes were up-regulated and 4435 genes were down-regulated. The results showed that the occurrence of CyHV-2 disease had a significant effect on the gene expression of intestinal mucosal tissue. The genes of cholesterol, bile acid biosynthesis pathway and protein were significantly differentially expressed in the diseased fish. The results showed that intestinal mucosal cholesterol and bile acid synthesis and metabolism were significantly affected. It was involved in the regulation of cholesterol, bile acid synthesis and metabolism, as well as cholesterol, bile acid secretion, absorption, transport and other physiological processes. The results showed that CyHV-2 disease had a serious effect on gene expression in intestinal mucosa and cholesterol. The synthesis and metabolism of bile acids and the gene expression of bile acid enterohepatic circulation pathway have a serious effect, which will lead to cholesterol in diseased fish. The content of bile acid in serum of fish with CyHV-2 disease decreased by 99%, and the content of cholesterol decreased by 10%, which confirmed the trend of differentially expressed genes.
【作者单位】：苏州大学基础医学与生物科学学院江苏省水产动物营养重点试验室;北京营养源研究所水产动物系统营养研究开放试验室;
【基金】：江苏省水产三新工程项目(D2015-12)资助~~
【分类号】：S943
【正文快照】： 油脂是水产动物饲料的主要营养物质和能量物质,而油脂易氧化,其氧化的中间产物、终产物对水产动物具有氧化损伤作用[1,2]。在草鱼饲料中添加氧化鱼油[3]或者对草鱼灌胃氧化鱼油[4],导致草鱼肝胰脏、肠道黏膜胆固醇生物合成通路基因差异表达,血清、肠道内胆汁酸含量显著下降;胆

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