六种常见牧草Matk基因DNA条形码研究

发布时间：2018-01-25 02:00

本文关键词： Matk基因牧草 DNA条形码鉴别　出处：《中兽医医药杂志》2016年01期 　论文类型：期刊论文

【摘要】：采用DNA条码(DNA Barcoding)技术对紫草科(聚合草)、豆科(紫花苜蓿、箭{H豌豆)和禾本科(羊茅、芨芨草和针茅)等6种牧草Matk基因的3个核苷酸片段(Matk1、Matk2、Matk3)进行测定和分析。结果表明,由于碱基插入(缺失),Matk1和Matk2各存在3种长度类型(L1~(259)、L1~(260)、L1~(262)及L2~(245)、L2~(246)、L2~(247)),Matk3存在2种长度类型(L3~(149)和L3~(150));因碱基变异,Matk1、Matk2和Matk3分别有4个(H1~A、H1~B、H1~C和H1~D)、3个(H2~A、H2~B和H2~C)和2个(H3~A和H3~B)单倍类型。据此建立了由碱基变异和插入(缺失)片段组合而成的6种牧草Matk基因识别码。根据碱基变异和插入(缺失)位点颜色差异及其所占据的相对位置,制作了DNA彩色条形码,通过比较纵向位置上的颜色差异实现6种牧草的鉴别。
[Abstract]:The DNA barcode Barcodingtechnique was used to study the effects of Artemiaceae (Alfalfa, Arrow {H Pea) and Gramineae (Festuca fescue) on Lymphaceae (Polygonatum), legume (Alfalfa, H pea). The three nucleotide fragments of Matk gene of six forage species, Achnatherum splendens and Stipa splendens, were determined and analyzed. The results showed that the Matk gene was inserted (missing). In Matk1 and Matk2, there were three length types: L1 / 259U / L1 / 260/ L1 / 262and L2 / 245/ L2 / 246respectively). There are two length types, L _ 3 and L _ (3), and L _ (3) ~ (1) and T _ (3) T _ (3), respectively, in L _ 2 ~ (2 +) ~ (247) and Matk3. Because of the base mutation, Matk1, Matk2 and Matk3 had four AH1, H1, BH1, and 3 H2H2A, respectively. The haplotypes of H _ 2o _ B and H _ 2C and H _ 3H _ 3A and H _ 3O _ B) haplotypes have been established based on which the base mutation and insertion (deletion) have been established. The Matk gene identification codes of 6 species of forage grass were composed of fragments. According to the color difference of base mutation and insertion (deletion) site and the relative position they occupied. The DNA color bar code was made and the color difference in longitudinal position was compared to realize the identification of 6 species of forage grass.
【作者单位】：甘肃农业大学草业学院草业生态系统教育部重点实验室(甘肃农业大学)中-美草地畜牧业可持续发展研究中心;甘肃农业大学动物科学技术学院;
【基金】：甘肃省高等学校科研项目(2013A-064)
【分类号】：S54
【正文快照】： DNA条形码是通过一段或几段通用的标准DNA序列对物种进行识别和鉴定的技术[1-3],从分子水平上对物种进行鉴定,为研究群落中物种网络提供了手段。在动植物间的植食网络关系中,当观察动物的行为存在困难时,可利用动物消化道中的食物残渣,借助DNA条形码技术分析其采食的植物,研究

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