痛泻要方对溃疡性结肠炎大鼠结肠黏膜STAT3基因和蛋白表达的影响

发布时间：2018-01-25 03:43

本文关键词： 痛泻要方溃疡性结肠炎白介素- STAT 蛋白表达基因表达　出处：《中华中医药杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的:观察痛泻要方对溃疡性结肠炎(UC)模型大鼠血清白介素-6(IL-6)、结肠黏膜STAT3蛋白和基因表达的影响,探讨其作用机制。方法:受试动物随机分为空白组,模型组,痛泻要方高、中、低剂量组及美沙拉秦(5-ASA)组,每组15只,痛泻要方低、中、高剂量组分别按生药1.4、2.8、5.6g/kg剂量灌胃,美沙拉秦组给药剂量为0.5g/kg,通过测定各组大鼠血清IL-6浓度、结肠组织STAT3蛋白和基因水平,探索痛泻要方对UC的干预机制。结果:与空白组比较,模型组大鼠血清IL-6含量、结肠组织STAT3基因和蛋白的表达量均明显升高(P0.01);治疗后,痛泻要方高剂量组与美沙拉秦组血清IL-6含量、STAT3基因和蛋白的表达量较模型组均明显降低(P0.05,P0.01)。结论:痛泻要方对肝郁脾虚型UC大鼠模型血清IL-6含量、结肠黏膜STAT3基因和蛋白的表达有下调作用,提示痛泻要方治疗UC的作用可能与抑制或阻断IL-6/JAK/STAT3信号转导途径有关。
[Abstract]:Objective: to observe the effect of Tongxieyao recipe on the expression of STAT3 protein and gene in serum interleukin-6 (IL-6) and colonic mucosa of rats with ulcerative colitis (UC). Methods: the experimental animals were randomly divided into blank group, model group, Tongxieyoufang high, middle and low dose groups and mezaladine 5-ASA-group with 15 rats in each group. The rats in the high dose group were given orally at the dose of 1.4g / kg 1.4g / kg and 2.8g / kg respectively, and the dosage of the drug was 0.5g / kg in the mesalazin group. The serum IL-6 concentration was measured by measuring the concentration of serum IL-6 in the rats of each group. The level of STAT3 protein and gene in colon tissue to explore the intervention mechanism of Tongxieyao recipe on UC. Results: compared with the blank group, the serum IL-6 content of the model group was higher than that of the control group. The expression of STAT3 gene and protein in colonic tissue was significantly higher than that in control group (P 0.01). After treatment, the expression of serum IL-6 and STAT3 gene and protein in high dose group and Mesalazin group were significantly lower than those in model group (P0.05). Conclusion: Tongxieyao recipe can down-regulate the content of serum IL-6 and the expression of STAT3 gene and protein in colon mucosa of UC rats with liver stagnation and spleen deficiency. The results suggest that the effect of Tongxieyiefang on UC may be related to the inhibition or blocking of IL-6/JAK/STAT3 signal transduction pathway.
【作者单位】：甘肃中医药大学;
【基金】：国家自然科学基金项目(No.81460696)~~
【分类号】：R285.5
【正文快照】： 溃疡性结肠炎(ulcerative colitis,UC)是一种易反复发作、缠绵难愈的炎性肠病,有癌变倾向,发病机制较为复杂[1],目前缺乏特效治疗方法,近些年发病率有明显升高趋势[2]。有研究表明[3],精神心理因素在消化系统疾病的发生、发展中起到重要作用,情志因素是影响UC发病与复发的关键

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