细胞周期调控基因及Bcl-2、c-Jun基因在人肝细胞脂肪变性过程中的表达变化

发布时间：2018-01-26 05:22

本文关键词： 肝细胞脂肪变性细胞周期细胞周期蛋白细胞周期蛋白依赖性激酶细胞周期蛋白依赖性激酶抑制因子 B细胞白血病-淋巴瘤基因 c-Jun基因　出处：《山东医药》2017年37期 　论文类型：期刊论文

【摘要】：目的观察细胞周期调控基因及Bcl-2、c-Jun基因在人肝细胞L02脂肪变性过程中的表达变化。方法以油酸和软脂酸(2∶1)混合液处理L02细胞0、5、10、20 h,建立人肝细胞脂肪变性模型。用油红O染色检测L02细胞内脂质含量;用荧光定量PCR技术检测L02细胞脂肪变性过程中CDK2、CDK4、Cyclin D1、Bcl-2、c-Jun mRNA表达。结果随造模时间延长,L02细胞内橘红色脂滴聚集越来越多,至造模20 h,肝细胞染成橘红色。L02细胞内CDK2 mRNA相对表达量逐渐升高,造模5 h达最高(P均0.05),随后逐渐下降;CDK4 mRNA相对表达量逐渐升高,造模10 h达最高(P均0.05),随后逐渐下降,造模20 h最低(P均0.05);不同时间点Cyclin D1 mRNA相对表达量比较差异无统计学意义(P均0.05);Bcl-2 mRNA相对表达量随造模时间延长而升高,造模10 h达最高(P0.05),随后降低;造模5、10、20 h的c-Jun mRNA相对表达量均较造模0 h高(P均0.05),但无变化规律。结论人肝细胞L02脂肪变性过程中癌症相关基因CDK2、CDK4、Bcl-2、c-Jun表达先升高后降低,Cyclin D1表达变化不明显。
[Abstract]:Objective to observe cell cycle regulation genes and Bcl-2. Methods the expression of c-Jun gene in L02 cells was treated with oleic acid and palmitate (2: 1) for 20 h. The fatty degeneration model of human hepatocytes was established and the lipid content in L02 cells was detected by oil red O staining. Fluorescence quantitative PCR technique was used to detect CDK2, CDK4, Cyclin D1 and Bcl-2 during fatty degeneration of L02 cells. Results the concentration of orange lipid droplets in L02 cells increased with the prolongation of modeling time, and the concentration of orange lipid droplets increased to 20 h. The relative expression of CDK2 mRNA in hepatocytes stained with orange-red. L02 increased gradually, reached the highest level at 5 h, and then decreased gradually. The relative expression of CDK4 mRNA increased gradually and reached the highest level at 10 h, then decreased gradually, and the lowest P was 0.05% at 20 h. There was no significant difference in the relative expression of Cyclin D1 mRNA at different time points (P < 0.05). The relative expression of Bcl-2 mRNA increased with the prolongation of modeling time, and reached the highest level at 10 h, and then decreased. The relative expression of c-Jun mRNA was significantly higher than that at 0 h (P 0.05). Conclusion the expression of cancer related gene CDK2pCDK4 / Bcl-2nc-Jun increased at first and then decreased during fatty degeneration of human hepatocytes L02. There was no obvious change in Cyclin D1 expression.
【作者单位】：南方医科大学南方医院·广东省南方消化病研究所;北京军事医学科学院微生物流行病研究所;
【基金】：国家高技术研究发展计划(863计划)(2015AA020702)
【分类号】：R575.5
【正文快照】： 2北京军事医学科学院微生物流行病研究所)脂肪肝是最常见的与代谢紊乱相关的慢性肝病,在全世界发病率高,约为11%~46%,并有潜在的严重后遗症[1]。脂肪肝疾病谱包括单纯性脂肪肝、脂肪性肝炎、肝纤维化、肝硬化和肝细胞癌。细胞周期分为G1、S、G2、M期。细胞周期转换主要受细胞

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