CYP2C9、CYP2A6基因多态性对丙戊酸钠血药浓度的影响

发布时间：2018-01-26 05:37

本文关键词： 丙戊酸钠 CYPC CYPA 基因多态性血药浓度　出处：《南京医科大学学报(自然科学版)》2017年01期 　论文类型：期刊论文

【摘要】：目的:研究CYP2C9、CYP2A6基因多态性对癫痫患儿丙戊酸钠血药浓度的影响。方法:收集单用丙戊酸钠治疗的癫痫患儿,应用荧光偏振免疫法测定丙戊酸钠血药浓度;分别采用直接测序法、巢式PCR法检测CYP2C9、CYP2A6的基因型;应用单因素方差分析研究基因多态性对丙戊酸钠血药浓度的影响。结果:83例癫痫患儿根据CYP2C9、CYP2A6基因型分为3组:强代谢组(CYP2C9*1*1合并CYP2A6*1*1)、中代谢组(CYP2C9*1*3合并CYP2A6*1*1或CYP2C9*1*1合并CYP2A6*1*4)和弱代谢组(CYP2C9*1*3合并CYP2A6*1*4或CYP2A6*4*4),3组分布频率分别为73.5%、24.1%和2.4%。中代谢组的标准化血药浓度显著高于高代谢组(P0.05),低代谢组的标准化血药浓度均数比其他两组高,但差异无统计学意义(P0.05)。结论:CYP2C9、CYP2A6的基因多态性能影响丙戊酸钠血药浓度,可通过检测基因型选择合适的丙戊酸钠初始剂量。
[Abstract]:Objective: To study the effects of CYP2C9, CYP2A6 gene polymorphism on serum concentration of valproic acid in children with epilepsy. Methods: single with sodium valproate in the treatment of children with epilepsy, blood concentration of sodium valproate was determined by fluorescence polarization immunoassay; respectively by direct sequencing and detection of CYP2C9 gene by nested PCR, CYP2A6; using single factor analysis of variance effect study on gene polymorphism on serum concentration of sodium valproate. Results: 83 cases of children with epilepsy according to CYP2C9, CYP2A6 genotypes were divided into 3 groups: Group (CYP2C9*1*1 and CYP2A6*1*1) metabolism, metabolic group (CYP2C9*1*3 combined with CYP2A6*1*1 or CYP2C9*1*1 combined with CYP2A6*1*4 group (CYP2C9*1*3) and weak metabolism with CYP2A6*1*4 or CYP2A6*4*4), the distribution of the 3 groups the frequency was 73.5%, blood drug concentration standard metabolism and 2.4%. in group 24.1% was significantly higher than that of high metabolic group (P0.05), blood drug concentration standard low metabolism group were the ratio of the number of The two groups were high, but the difference was not statistically significant (P0.05). Conclusion: the polymorphism of CYP2C9 and CYP2A6 affects the plasma concentration of valproate, and the appropriate initial dosage of valproate can be selected by detecting genotypes.

【作者单位】：南京医科大学附属儿童医院药学部;
【基金】：南京医科大学科技发展基金面上项目(2013NJMU117)
【分类号】：R969
【正文快照】： 丙戊酸钠(sodium valproate,VPA)是对多种类型癫痫有效的一线广谱抗癫痫药,在临床上应用广泛,其治疗指数低、安全范围窄、体内过程和疗效个体差异大。VPA的有效血药浓度参考范围为50~100 mg/L,其不良反应与血药浓度密切相关,因此对用药儿童进行血药浓度监测十分重要[1]。VPA大

【相似文献】