HLA-B27抗原检测与基因检测在强直性脊柱炎诊断中的应用

发布时间：2018-01-26 11:21

本文关键词： 强直性脊柱炎人类白细胞抗原B(HLA-B) 流式细胞术基因亚型　出处：《细胞与分子免疫学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的研究强直性脊柱炎(AS)中HLA-B27抗原表达与基因亚型分布特点,探讨HLA-B27抗原和基因检测在AS诊断中的应用价值。方法收集103例AS患者及105例健康人群对照静脉全血,分别采用流式细胞术(FCM)及基于序列分析的PCR分型法(PCR-SBT)检测HLA-B27。结果 FCM检测HLA-B27诊断AS的灵敏度和特异度分别为97.09%和92.38%,PCR-SBT诊断AS的灵敏度和特异度为96.12%和97.14%。AS组HLA-B位点等位基因检出频率以B27最高。结论 HLA-B27抗原和基因检测对AS诊断均具有较高价值。FCM检出灵敏度略高于PCR-SBT,但特异度低于PCR-SBT。
[Abstract]:Objective to study the expression of HLA-B27 antigen and the distribution of gene subtypes in ankylosing spondylitis (ASA). To evaluate the value of HLA-B27 antigen and gene detection in the diagnosis of as. Methods whole venous blood samples were collected from 103 patients with as and 105 healthy controls. Flow cytometry (FCM) and PCR typing based on sequence analysis (PCR-SBT) were used respectively. Results the sensitivity and specificity of FCM in the diagnosis of as by HLA-B27 were 97.09% and 92.38%, respectively. The sensitivity and specificity of PCR-SBT in the diagnosis of as were 96.12% and 97.14% respectively. The frequency of allele detection at HLA-B locus in as group was the highest in B27. The sensitivity of HLA-B27 antigen and gene detection for as diagnosis was slightly higher than that of PCR-SBT. But the specificity was lower than that of PCR-SBT.
【作者单位】：福建医科大学附属第二医院中心实验室;福建医科大学附属第二医院东海检验科;
【基金】：泉州市科技项目(Z[2014]0008)
【分类号】：R593.23
【正文快照】： 强直性脊柱炎(ankylosing spondylitis,AS)是一种病因未明的慢性进行性炎症疾病[1],以脊柱及骶髂关节受累为主要临床表现。在中国,AS的患病率约为0.3%,在炎症性关节炎中居第二位[2-3]。人类白细胞抗原B27(human leukocyte antigen B27,HLA-B27)与AS相关[4-5],是临床辅助诊断AS

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